Strip for lateral flow assay, comprising subpad, and cartridge for lateral flow assay used for the same
The present application relates to: a strip for lateral flow assay, comprising a support, a medium for development, a sample pad comprising a subpad, and an absorption pad; and a cartridge for lateral flow assay, comprising the same. According to the present application, the strip adopts a novel sample loading method, thereby obtaining reproducible results irrespective of the volume of an injected sample, and it is possible to readily change the amount of a sample, as necessary, thereby improving the reproducibility of measured results and convenience.
1. A cartridge for a lateral flow assay accommodating a strip comprising:
a base member comprising a strip receiving part accommodating the strip, and a sample receiving well extended from the strip receiving part formed at one end of the base member; and
a cover member comprising a sample inlet and a measurement window, the cover member covering the base member,
wherein the sample receiving well is formed at a position that is corresponds to the sample inlet when the cover member covers the base member,
wherein the strip comprises a solid support, a chromatographic medium, a sample pad with a subpad comprising a body portion, a slant extended from the body portion and a dipping portion extended from the slant, and an absorption pad; the chromatographic medium, the sample pad and the absorption pad being positioned on the solid support; the sample pad and the absorption pad each being positioned on each end of the solid support with the chromatographic medium located therebetween in such a way that one end of the chromatographic medium is adjacent to one end of the sample pad and the other end of the chromatographic medium is adjacent to one end of the absorption pad;
wherein the cover member comprises three guides formed at a second position, a third position and a fourth position inside the cover member facing the strip, the second positon corresponding to an area where the boundary of the slant and the body portion of the subpad is located when the cover member covers the base member; the third position corresponding to an area where the boundary of the sample pad and the body portion of the subpad is located when the cover member covers the base member; and the fourth position corresponding to an area where the boundary of the sample pad and the chromatographic medium is located when the cover member covers the base member.
2. The cartridge of claim 1 , further comprising a guide formed at a first position inside the cover member facing the strip, the first position corresponding to an area of the sample receiving well where the slant of the subpad is located when the cover member covers the base member.
3. The cartridge of claim 1 , wherein the guides takes the form of a dam or a protrusion.
4. The cartridge of claim 1 , wherein the cover member further comprises a ventilation window located between the sample inlet and the measurement window.
5. The cartridge of claim 1 , further comprising a cover for the measurement window for opening or closing the measurement window.