Oral delivery of nucleic acid-based gene interfering agents by
The present invention provides vectors, including a novel attenuated strain of Salmonella , for efficient gene transfer into an animal, e.g. a mammalian host.
1. A cell infected with the virus with an attenuated Salmonella having a deletion in ssrA/B comprising a plasmid construct encoding a targeted M1GS ribozyme that cleaves viral mRNA, wherein viral replication is inhibited.
2. The cell of claim 1 , wherein the ribozyme is a functional RNase P-based ribozyme.
3. An attenuated Salmonella having a deletion in ssrA/B comprising a plasmid construct encoding a targeted M1GS ribozyme that cleaves viral mRNA, wherein viral replication is inhibited.
4. The cell of claim 1 , wherein the virus is a cytomegalovirus.
5. The cell of claim 1 , wherein the M1GS ribozyme is targeted to a region accessible to ribozyme binding.
6. The cell of claim 1 , wherein the attenuated Salmonella is SL101.