Renal function analysis method and apparatus
A method for measuring a glomerular filtration rate in a mammalian kidney comprises a source of reporter and marker fluorescent molecules. The fluorescent molecules are introduced into the blood stream of a mammalian subject. Over a period of time, a measurement of the intensities of the reporter and marker fluorescent molecules is taken. A ratio is calculated to determine the health of the subject's kidney. This method measures volume of plasma distribution based on a fluorescence of a marker molecule relative to a fluorescence of a reporter molecule.
1. An apparatus for analyzing the condition of a kidney, said apparatus comprising:
a source of an analysis composition, said analysis composition comprising a plurality of fluorescent reporter molecules and a plurality of fluorescent marker molecules, said reporter molecules and said marker molecules sharing a molecular property;
a percutaneously introducible catheter configured for introducing the analysis composition into a blood steam of a patient and for in situ delivery of excitation energy from an optical fiber extensible from a distal end of the catheter into the bloodstream of the patient and for collection of emission energy from within the bloodstream of the patient; and
a detector for monitoring a level of the analysis composition within the blood stream and reporting the operating condition of the kidney;
wherein a volume of plasma distribution of the patient is calculated based on the fluorescence of the marker molecules, and
wherein the fluorescence of the marker molecules relative to the fluorescence of the reporter molecules is calculated and reported as a measure of kidney function.
2. The apparatus of claim 1 wherein the molecular property is selected from the group consisting of molecular weight, size, shape, charge, fluorescence and radio frequency.
3. The apparatus of claim 2 wherein the molecular property is fluorescence, the reporter molecule has a first fluorescence, and the marker molecule has a second fluorescence.
4. The apparatus of claim 2 wherein the molecular property is molecular weight, the reporter molecule has a first molecular weight, and the marker molecule has a second molecular weight which is greater than the molecular weight of the reporter molecule.
5. The apparatus of claim 4 wherein the reporter molecule is readily filtered by the kidney, and the marker molecule resists filtration by the kidney.
6. The apparatus of claim 5 wherein the first molecular weight is between about 3 kD and 70 kD.
7. The apparatus of claim 6 wherein the first molecular weight is between about 3 kD and 20 kD.
8. The apparatus of claim 1 wherein the reporter molecule and marker molecules are both dextrans.
9. The apparatus of claim 8 wherein the dextrans are conjugated with fluorescence.
10. The apparatus of claim 8 wherein the reporter molecule is fluorescein dextran.
11. The apparatus of claim 8 wherein the reporter molecule is a fluorescein isothiocyanate-inulin.
12. The apparatus of claim 8 wherein the marker molecule is a larger sulforhodamine 101 dextran that is not filtered by the kidney.
13. The apparatus of claim 1 wherein the fiber optic cable has a diameter of from about 0.5 mm to about 1.0 mm.
14. The apparatus of claim 1 wherein the detector is selected from the group consisting of photo multiplier tubes, photo detectors, solid state detectors, a charge-coupled device, and combinations thereof.
15. The apparatus of claim 14 wherein the detector includes at least one LED source or laser diode, at least one optical filter, and at least one power supply.
16. The apparatus of claim 15 wherein the detector further comprises one of a dichroic and band pass filter.
17. The apparatus of claim 16 wherein the detector comprises a microcontroller.
18. The apparatus of claim 1 wherein the apparatus measures the glomerular filtration rate (GFR) of the kidney.
19. An apparatus for analyzing the condition of a kidney, said apparatus comprising:
a source of a kidney function analysis composition, said composition comprising a plurality of fluorescent reporter molecules and a plurality of fluorescent marker molecules, said reporter molecules and said marker molecules sharing a molecular property;
a percutaneously introducible catheter comprising:
a tubular main member defining a passageway between a proximal end and a distal end thereof, and
an optical fiber extensible from the distal end; and
a detector, optically coupled to the optical fiber, for monitoring a level of the analysis composition within the blood stream and reporting vascular plasma volume and the operating condition of the kidney;
wherein the volume of plasma distribution is calculated based on the fluorescence of the marker molecules and the fluorescence of the marker molecules relative to the fluorescence of the reporter molecules is calculated and reported as a measure of kidney function and condition.
20. The apparatus of claim 19 wherein said optical fiber is slidable within the passageway.
21. The apparatus of claim 19 wherein said optical fiber includes a bend proximate a distal end thereof.
22. The apparatus of claim 19 wherein said optical fiber is configurable for in situ delivery of excitation energy and collection of emission energy within the bloodstream a patient.
23. The apparatus of claim 19 wherein said catheter is configured for introducing the analysis composition into the blood steam of a patient.