IP Library Granted Patent US 9,400,377
Granted Patent B2
US 9,400,377 · App. 13/498,912 · Granted Jul 26, 2016

Automatic focusing method for an optical instrument for magnified viewing of an object

Inventor: Markus Eichinger (Tuerkenfeld, DE)
Assignee: Carl Zeiss Microscopy GmbH
G02B21/244G02B7/36G02B27/0075
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Quick Facts
Patent No.
US 9,400,377
App. No.
13/498,912
Granted
Jul 26, 2016
Kind
B2
Abstract

A focusing method for an optical instrument for magnified viewing of an object includes producing at least two images with different focal positions and applying several different sharpness functions. Each function has an absolute extremum which specifies the optimum focal position to be set for the viewing and which lies in a close focus range of the sharpness function adjoined on both sides by a far focus range which contains local secondary extrema. For each of the at least two images an evaluation thereof is performed with the focal position as a variable such that a sharpness function variation direction of the focal position is calculated for each sharpness function in order to arrive at the optimum focal position. A determination is made for main variation direction based on all sharpness function variation directions, which is then used during further focusing.

Claims (25)

1. A method of focusing an optical instrument for magnified viewing of an object, the optical instrument comprising instrument optics, an adjusting unit for adjusting the focal position when viewing the object by means of the instrument optics, and a recording unit which records the object by means of the instrument optics, the method comprising the steps of:

a) producing at least two images with different focal positions;

b) applying several different sharpness functions to each of the at least two images, each of the several different sharpness functions having an absolute extremum which specifies the optimum focal position to be set for the viewing and which lies in a close focus range of the sharpness function adjoined on both sides by a far focus range which is more susceptible to interferences during recording than the close focus range and therefore contains local secondary extrema, with the focal position as a variable such that a sharpness function variation direction of the focal position is calculated for each sharpness function in order to arrive at the optimum focal position;

c) determining a main variation direction by simultaneously utilizing together all sharpness function variation directions such that the main variation direction is calculated based on all of the sharpness function variation directions; and

d) using the determined main variation direction during further focusing.

2. The method according to claim 1 , wherein the sharpness function variation direction in step b) is determined by means of a gradient calculation.

3. The method according to claim 2 , wherein, in step c) the main variation direction is determined by a majority decision or a weighted majority decision from among all sharpness function variation directions.

4. The method according to claim 3 , wherein, in step c) it is ascertained, based on all sharpness function variation directions, whether the focal positions of the at least two images lie in one of the far focus range and in the close focus range, and this is taken into account during further focusing.

5. The method according to claim 4 , wherein, during further focusing, the step size for changing the focal position for further images is set higher if the far focus range has been ascertained compared with the case where the close focus range has been ascertained.

6. The method according to claim 2 , wherein, in step c) it is ascertained, based on all sharpness function variation directions, whether the focal positions of the at least two images lie in the far focus range or in the close focus range, and this is taken into account during further focusing.

7. The method according to claim 6 , wherein, during further focusing, the step size for changing the focal position for further images is set higher if the far focus range has been ascertained compared with the case where the close focus range has been ascertained.

8. The method according to claim 1 , wherein, in step c) the main variation direction is determined by one of a majority decision and a weighted majority decision from among all sharpness function variation directions.

9. The method according to claim 8 , wherein, in step c) it is ascertained, based on all sharpness function variation directions, whether the focal positions of the at least two images lie in one of the far focus range and in the close focus range, and this is taken into account during further focusing.

10. The method according to claim 9 , wherein, during further focusing, the step size for changing the focal position for further images is set higher if the far focus range has been ascertained compared with the case where the close focus range has been ascertained.

11. The method according to claim 1 , wherein, in step c) it is ascertained, based on all sharpness function variation directions, whether the focal positions of the at least two images lie in one of the far focus range and in the close focus range, and this is taken into account during further focusing.

12. The method according to claim 11 , wherein, during further focusing, the step size for changing the focal position for further images is set higher if the far focus range has been ascertained compared with the case where the close focus range has been ascertained.

13. An optical instrument for magnified viewing of an object, comprising:

instrument optics;

an adjusting unit for adjusting the focal position when viewing the object by means of the instrument optics,

a recording unit which records the object by means of the instrument optics; and

an evaluation and control unit, the evaluation and control unit configured:

to be fed at least two images with different focal positions;

to apply several different sharpness functions to each of the at least two images, each of the several different sharpness functions having an absolute extremum which specifies the optimum focal position to be set for the viewing and which lies in a close focus range of the sharpness function adjoined on both sides by a far focus range which is more susceptible to interferences during recording than the close focus range and therefore contains local secondary extrema, in each case to each of the at least two images, and evaluate them with the focal position as a variable such that for each sharpness function a sharpness function variation direction of the focal position is calculated in order to arrive at the optimum focal position;

to determine a main variation direction by simultaneously utilizing together all sharpness function variation directions such that the main variation direction is calculated based on all of the sharpness function variation directions; and

to use the determined main variation direction during further focusing.

Assignments (2)
CHANGE OF NAME Recorded Jun 18, 2013
From: CARL ZEISS MICROIMAGING GMBH
To: CARL ZEISS MICROSCOPY GMBH
Reel/Frame 030629/0113 →
ASSIGNMENT OF ASSIGNOR'S INTEREST Recorded Mar 28, 2012
From: EICHINGER, MARKUS, DR.
To: CARL ZEISS MICROIMAGING GMBH
Reel/Frame 027950/0083 →
Priority Claims (1)
DE 10 2009 045 107 · Sep 29, 2009 · national
Continuity (1)
Related Publication 20120218460A1 · Aug 30, 2012