Assay cartridges and methods of using the same
Assay modules, preferably assay cartridges, are described as are reader apparatuses which may be used to control aspects of module operation. The modules preferably comprise a detection chamber with integrated electrodes that may be used for carrying out electrode induced luminescence measurements. Methods are described for immobilizing assay reagents in a controlled fashion on these electrodes and other surfaces. Assay modules and cartridges are also described that have a detection chamber, preferably having integrated electrodes, and other fluidic components which may include sample chambers, waste chambers, conduits, vents, bubble traps, reagent chambers, dry reagent pill zones and the like. In certain preferred embodiments, these modules are adapted to receive and analyze a sample collected on an applicator stick.
1. An apparatus for performing a plurality of biochemical assays comprising:
a plurality of electrodes comprising at least one dedicated working electrode, at least one dual-role electrode and at least one dedicated counter electrode, said dedicated working and dual-role electrodes having deposited thereon an assay reagent, wherein said plurality of electrodes are arranged within a flow cell, said flow cell having a flow cell path, and wherein said dual-role electrode is configured to operate first as the working electrode and then as the counter electrode;
dedicated working, dual-role and dedicated counter electrical leads for supplying electrical energy to said plurality of electrodes, wherein at least two non-adjacent electrical leads have an exposed surface located thereon;
an inlet conduit in fluid communication with said flow cell and at least partially defined by said exposed surfaces of said electrical leads, wherein fluid in said inlet conduit is in electrical contact with said exposed surfaces, and
wherein the exposed surfaces are configured to apply an inlet conduit interrogation potential between said exposed surfaces to determine the presence or composition of fluid in said inlet conduit.
2. The apparatus according to claim 1 wherein said assay reagent is a binding reagent specific for an analyte of interest.
3. The apparatus according to claim 2 wherein said binding reagent is different for each of said dedicated working and dual-role electrodes.
4. The apparatus according to claim 1 wherein said plurality of electrodes are arranged along said flow cell path.
5. The apparatus according to claim 1 wherein said dedicated counter electrode is adjacent said dual-role electrode and said dual-role electrode is adjacent said dedicated working electrode.
6. The apparatus according to claim 1 wherein said plurality of electrodes are arranged within a single detection chamber.
7. The apparatus according to claim 1 wherein said plurality of electrodes are printed carbon ink.
8. The apparatus according to claim 2 wherein said assay reagents are present within an assay domain defined by a dielectric layer on said dedicated working and dual-role electrodes.
9. The apparatus according to claim 1 wherein said exposed surfaces are further configured such that the applied interrogation potential is of insufficient magnitude to induce electrochemiluminescence at the corresponding electrodes.
10. The apparatus according to claim 1 further comprising an optical detector for detecting luminescence generated at said dedicated working and dual-role electrodes.
11. The apparatus according to claim 1 further comprising a voltmeter for measuring potentials at said dedicated working and dual-role electrodes.
12. The apparatus according to claim 1 further comprising an ammeter for measuring electrical current at said dedicated working and dual-role electrodes.