Cell-derived composition
The invention is directed to a cell-derived composition having bone growth, regeneration, and repair promoting properties. In particular, the invention is directed to a cell-derived composition having bone growth, regeneration, and repair promoting properties termed Amnion-derived Cellular Cytokine Solution-B (ACCS-B). The invention is further directed to the use of this composition to promote bone growth and/or regeneration and/or repair.
1. A composition comprising Amnion-derived Cellular Cytokine Solution-B (ACCS-B), wherein the composition is made by a method comprising the step of culturing Amnion-derived Multipotent Progenitor (AMP) cells in Basal Medium Eagle (BME) culture medium supplemented with human serum albumin and recombinant human EGF.
2. The composition of claim 1 which is a pharmaceutical composition.
3. The composition of claim 1 which is formulated for sustained-release (SR).
4. A method for promoting bone growth comprising administering to a subject in need thereof a therapeutically effective dose of a composition selected from the group consisting of ACCS-B and SR-ACCS-B.
5. A method for making ACCS-B comprising the step of culturing Amnion-derived Multipotent Progenitor (AMP) cells in Basal Medium Eagle (BME) culture medium supplemented with human serum albumin and recombinant human EGF.