Small molecule antagonists of bacterial quorum-sensing receptors
A novel small molecule antagonizes two types of acyl homoserine lactone receptors: membrane-bound and cytoplasmic. A focused library of analogs and derivatives of the original antagonist was synthesized. Analog and derivative molecules harbor a range of activities. The novel small molecule and most potent antagonist protects the eukaryote Caenorhabditis elegans from quorum-sensing-mediated killing by the bacterial pathogen Chromobacterium violaceum . The saving of C. elegans demonstrates the use of these molecules as small molecule antimicrobials.
1. A pharmaceutical composition comprising a pharmaceutically acceptable carrier, excipient or diluent and one or more small molecules represented by the formula:
wherein n is 0, 1 or 2; Y is O, S or CH 2 ;
wherein the aryl ring substituent Z represents single or multiple substituents, wherein when Z represents a single substituent Z is located at the ortho, meta or para position and when Z represents multiple substituents, the substituents are located at any combination of the ortho, meta or para positions, and wherein Z is selected from the group consisting of halogen, hydroxyl, alkoxyl, cyano, nitro, amido, acetamido, amino, alkylamino, aryl, heteroaryl, acyl, alkyl, cycloalkyl, sulfonamide, and alkyl sulfonamide.
2. The pharmaceutical composition of claim 1 wherein the small molecules are:
3. A method for treating a bacterial infection by gram negative quorum sensing bacteria, comprising administering to a subject in need, a therapeutically effective amount of the pharmaceutical composition of claim 1 .
4. The method of claim 3 wherein the bacteria are Pseudomonas aeruginosa.
5. The method of claim 4 wherein the subject in need has a cystic fibrosis lung infection.