Outer membrane protein 18 as a diagnostic marker for campylobacter
Accurate and fast detection of the presence of Campylobacter disease is important for the proper treatment of patients with Campylobacter infection. Present tests depend upon culture of viable bacteria and identification by microscopy, which requires care, skill, and two or more days for conclusive results. The current invention improves the ease of use and overcomes the limitations of loss of viability and delay inherent in Campylobacter bacterial culture and provides a more rapid alternative for the identification and diagnosis of Campylobacter and campylobacteriosis. The invention provides a new method of detecting Campylobacter by utilizing an outer membrane protein (OMP 18) to develop antibodies for use in immunoassays of bacterial cultures or human fecal samples.
1. A method for testing a fecal sample from a person having symptoms associated with gastroenteritis, the method comprising:
obtaining a fecal sample from the person having symptoms associated with gastroenteritis;
using one or more antibodies against Outer Membrane Protein 18 (OMP18) to determine whether OMP18 is present in the fecal sample; and
upon determining OMP18 is present in the fecal sample, diagnosing the person with Campylobacter infection.
2. The method of claim 1 , wherein the one or more antibodies against OMP18 are raised against a full length sequence of a native OMP18 purified from at least one Campylobacter species.
3. The method of claim 1 , wherein the one or more antibodies against OMP18 are raised against recombinant OMP18 expressed in bacteria, yeast, or mammalian cells.
4. The method of claim 1 , wherein the one or more antibodies against OMP18 comprise one or more polyclonal antibodies that specifically recognize OMP18.
5. The method of claim 1 , wherein the one or more antibodies against OMP18 are raised against one or more OMP18 proteins from one or more Campylobacter species.
6. The method of claim 1 , wherein the presence of OMP18 is identified using lateral flow techniques and immunochemical reactions on a membrane or an enzyme-linked immunoassay.
7. The method of claim 6 , wherein the one or more antibodies against OMP18 are raised against a full length sequence of a native OMP18 purified from at least one Campylobacter species.
8. The method of claim 6 , wherein the one or more antibodies against OMP18 are raised against recombinant OMP18 expressed in bacterial, yeast, or mammalian cells.
9. The method of claim 6 , wherein the one or more antibodies against OMP18 are raised against one or more OMP18 proteins from one or more Campylobacter species.
10. The method of claim 6 , wherein the one or more antibodies comprise one or more polyclonal antibodies that specifically recognize OMP18.
11. The method of claim 1 , wherein the one or more antibodies against OMP18 comprise one or more monoclonal antibodies that specifically recognize OMP18.
12. The method of claim 6 , wherein the one or more antibodies against OMP18 comprise one or more monoclonal antibodies that specifically recognize OMP18.
13. The method of claim 1 , wherein the one or more antibodies against OMP18 are raised against a protein having at least 70% identity with SEQ. ID NO:1 purified from at least one Campylobacter species.
14. The method of claim 6 , wherein the one or more antibodies against OMP18 are raised against a protein having at least 70% identity with SEQ. ID NO:1 purified from at least one Campylobacter species.
15. The method of claim 1 , wherein the one or more antibodies against OMP18 are raised against a protein having at least 89% identity with SEQ. ID NO:1 purified from at least one Campylobacter species.
16. The method of claim 6 , wherein the one or more antibodies against OMP18 are raised against a protein having at least 89% identity with SEQ. ID NO:1 purified from at least one Campylobacter species.
17. The method of claim 1 , wherein the one or more antibodies against OMP18 are raised against a protein having at least 94% identity with SEQ. ID NO:1 purified from at least one Campylobacter species.
18. The method of claim 6 , wherein the one or more antibodies against OMP18 are raised against a protein having at least 94% identity with SEQ. ID NO:1 purified from at least one Campylobacter species.
19. The method of claim 5 , wherein the one or more OMP18 proteins comprise at least two OMP18 proteins from different Campylobacter species, the at least two OMP18 proteins having amino acid sequences that are at least partly different from one another.
20. The method of claim 1 , wherein the presence of OMP18 is identified using lateral flow techniques and immunochemical reactions on a membrane.