Selective cytopheresis devices and related methods thereof
View Patent ↗The present invention relates to systems and devices to treat and/or prevent inflammatory conditions within a subject and to related methods. More particularly, the invention relates to systems, devices, and related methods that sequester leukocytes and/or platelets and then inhibit their inflammatory action.
1. A system for treating activated platelets from a subject in need thereof, the system comprising:
a device comprising a housing defining a passageway that permits body fluid from the subject to pass therethrough and a fiber or a plurality of fibers disposed within the housing, wherein each fiber has an exterior surface that binds the platelets; and
a calcium chelating agent comprising citrate present in an amount sufficient to inhibit the release of a pro-inflammatory substance from the activated platelets or to deactivate the platelets.
2. The system of claim 1 , further comprising a second device in series with the device comprising the housing defining the passageway.
3. The system of claim 1 , wherein the calcium chelating agent is infused into the passageway.
4. The system of claim 1 , wherein the surface that binds the platelets comprises a membrane.
5. The system of claim 4 , wherein the membrane is porous.
6. The system of claim 5 , wherein the membrane permits the passage of fluid therethrough.
7. The system of claim 4 , wherein the membrane has a surface area greater than about 0.2 m 2 .
8. The system of claim 4 , wherein a surface of the membrane binds the platelets.
9. The system of claim 1 , wherein the device is configured such that shear force within the passageway is less than about 10 dynes/cm 2 when fluid passes through the passageway at a rate of from about 100 mL/minute to about 500 mL/minute.
10. The system of claim 1 , wherein the exterior surface that binds the platelets comprises a cell-adhesion molecule that binds an activated platelet.
11. The system of claim 1 , wherein the fiber is, or plurality of fibers are, hollow.
12. The system of claim 1 , wherein the device is cell-free.
13. The system of claim 1 , wherein the device is configured to bind the platelets for a time sufficient to permit the subsequent inhibition or release of a pro-inflammatory substance from the platelets and/or deactivation of the platelets.
14. The system of claim 1 , wherein the exterior surface of the fiber binds the platelets.