Automated intracytoplasmic sperm injection assisted fertilization system
An integrated automated system comprising a microfluidic cassette, and methods of use thereof, for intracytoplasmic sperm injection assisted fertilization. The microfluidic cassette and the integrated automated system provides a complete set-up of human gametes for assisted in vitro fertilization, including proper cell stage recognition, gamete propulsion via microfluidic currents, microinjection of a spermatozoon into an oocyte, and subsequent embryo culture and monitoring, thus allowing widespread distribution of in vitro insemination by favoring affordability.
1. An enclosed microfluidics cassette device for intracytoplasmic sperm injection assisted fertilization comprising:
a) an oocyte reservoir comprising an oocyte chamber, an inlet and an outlet;
b) an oocyte cumulus removal channel in selective fluid communication with the oocyte reservoir outlet;
c) an oocyte immobilizing station in selective fluid communication with the oocyte cumulus removal channel;
d) a spermatozoa reservoir comprising a spermatozoa chamber, an inlet and outlet;
e) a motile spermatozoa isolating channel in selective fluid communication with the spermatozoa reservoir outlet;
f) a motile spermatozoa immobilization station in selective fluid communication with the motile spermatozoa isolating channel and in selective fluid communication with the oocyte immobilization station; and
g) an embryo culturing chamber in selective fluid communication with the oocyte immobilization station.
2. The device of claim 1 , wherein a portion of the oocyte cumulus removal channel has a narrower width than the oocyte chamber allowing individual progression of an oocyte.
3. The device of claim 2 , wherein a portion of said oocyte cumulus removal channel has a width about 200 mm.
4. The device of claim 1 , wherein the oocyte cumulus removal channel has a 90° turn allowing rotation of an oocyte to remove cumulus cells.
5. The device of claim 1 , wherein selective fluid communication is controlled by one or more piezoelectric gates comprising nanoelectrode modules.
6. The device of claim 1 , wherein the oocyte cumulus removal channel has a waste outlet in selective fluid communication therewith.
7. The device of claim 1 , wherein the motile spermatozoa isolating channel has an immotile spermatozoa outlet in selective fluid communication therewith.
8. The device of claim 1 , further comprising a plurality of embryo culturing chambers in selective fluid communication with the oocyte immobilization station.
9. The device of claim 1 , further comprising one or more embryo observation stations in selective fluid communication with the embryo culturing chamber.
10. The device of claim 1 , further comprising one or more sperm observation stations in selective fluid communication with the motile spermatozoa immobilization station.
11. The device of claim 1 , further comprising one or more oocyte observation stations in selective fluid communication with the oocyte immobilizing station.
12. The device of claim 1 , further comprising a plurality of oocyte reservoirs in selective fluid communication with a plurality of oocyte cumulus removal channels in selective fluid communication with the oocyte immobilizing station.
13. The device of claim 1 , wherein the motile spermatozoa immobilization station comprises an intracytoplasmic sperm injection system.
14. The device of claim 1 , adapted for insertion into a machine for automated monitoring and control of oocyte cumulus removal, oocyte selection and immobilization, motile spermatozoa isolation, motile sperm immobilization, intracytoplasmic sperm injection, and embryo monitoring.
15. A system for automated intracytoplasmic sperm injection assisted fertilization comprising:
an enclosed microfluidics cassette device for intracytoplasmic sperm injection assisted fertilization comprising:
a) an oocyte reservoir comprising an oocyte chamber, an inlet and an outlet;
b) an oocyte cumulus removal channel in selective fluid communication with the oocyte reservoir outlet;
c) an oocyte immobilizing station in selective fluid communication with the oocyte cumulus removal channel;
d) a spermatozoa reservoir comprising a spermatozoa chamber, an inlet and outlet;
e) a motile spermatozoa isolating channel in selective fluid communication with the spermatozoa reservoir outlet;
f) a motile spermatozoa immobilization station in selective fluid communication with the motile spermatozoa isolating channel and in selective fluid communication with the oocyte immobilization station; and
g) an embryo culturing chamber in selective fluid communication with the oocyte immobilization station; and
a machine for functionally engaging the microfluidics cassette device comprising a central processing unit (CPU) and software for automated monitoring and control of selective communication for progression of microfluidics, oocyte cumulus removal, oocyte selection and immobilization, motile spermatozoa isolation, motile sperm immobilization, intracytoplasmic sperm injection, and embryo monitoring.
16. The system of claim 15 , wherein the machine further comprises a plurality of video cameras and remote video monitors connected to the CPU.
17. The system of claim 15 , wherein the CPU automatically controls the temperature of the device and the addition of cellular media to the oocyte reservoir inlet and spermatozoa reservoir inlet.
18. The system of claim 15 , wherein the machine controls the oocyte immobilizing station by creating negative pressure through a microchannel in the oocyte immobilizing station.
19. The system of claim 15 , wherein the machine controls the intracytoplasmic sperm injection by applying an electric field from a pizeo-pipette to penetrate the oocyte and then applying microfluidic pressure inside the pizeo-pipette to position the spermatozoa into the oocyte.
20. A method for automated intracytoplasmic sperm injection assisted fertilization comprising:
combining a spermatozoa and an oocyte in a system comprising:
an enclosed microfluidics cassette device for intracytoplasmic sperm injection assisted fertilization comprising:
a) an oocyte reservoir comprising an oocyte chamber, an inlet and an outlet;
b) an oocyte cumulus removal channel in selective fluid communication with the oocyte reservoir outlet;
c) an oocyte immobilizing station in selective fluid communication with the oocyte cumulus removal channel;
d) a spermatozoa reservoir comprising a spermatozoa chamber, an inlet and outlet;
e) a motile spermatozoa isolating channel in selective fluid communication with the spermatozoa reservoir outlet;
f) a motile spermatozoa immobilization station in selective fluid communication with the motile spermatozoa isolating channel and in selective fluid communication with the oocyte immobilization station; and
g) an embryo culturing chamber in selective fluid communication with the oocyte immobilization station; and
a machine for functionally engaging the microfluidics cassette device comprising a central processing unit (CPU) and software for automated monitoring and control of selective communication for progression of microfluidics, oocyte cumulus removal, oocyte selection and immobilization, motile spermatozoa isolation, motile sperm immobilization, intracytoplasmic sperm injection, and embryo monitoring.