Method for producing scyllo-inositol
The disclosure provides a method of producing a scyllo-inositol or a new scyllo-inositol derivative in a one-step process, from ubiquitous and inexpensive raw materials. Also provided is a scyllo-inositol derivative bonded to saccharides such as glucose and similar.
1. A method for producing scyllo-inositol and a scyllo-inositol derivative comprising:
preparing a transformed microorganism by transfecting a host microorganism with expression cassettes comprising an inositol-1-phosphoric acid synthase gene, inositol monophosphatase gene, myo-inositol dehydrogenase gene, and iolW gene to overexpress said genes in the transformed microorganism, wherein the host microorganism is Escherichia coli ; and
contacting the transformed microorganism with glucose or disaccharides or polysaccharides having glucose units under conditions suited to the growth and/or maintenance of the transformed microorganism.
2. The production method according to claim 1 wherein the scyllo-inositol derivative is a compound shown by the following structural formula:
3. The method according to claim 1 , wherein said expression cassettes comprises first, second, third and fourth expression cassettes comprising said inositol-1-phosphoric acid synthase gene, said inositol monophosphatase gene, said myo-inositol dehydrogenase gene, and said iolW gene, respectively.
4. The method according to claim 1 , wherein said inositol monophosphatase gene is a suhB gene.
5. The method according to claim 1 , wherein said inositol-1-phosphoric acid synthase gene is a INO1 gene.