Mechanical cell lysis apparatus
View Patent ↗The present invention relates to a cell lysis apparatus and a manufacturing method thereof, and more particularly, to a cell lysis apparatus, which mechanically performs cell lysis, and a manufacturing method thereof. The cell lysis apparatus includes: an inlet port through which fluid containing cells is supplied; a fluid channel defining a passage through which the cell containing fluid supplied from the inlet port flows; a nano blade array including a plurality of nano blades having pointed leading ends and arranged such that the pointed leading ends of the nano blades are oriented in a direction of the fluid supplied through the inlet port to disrupt the cells passing therethrough; and an outlet port through which the disrupted cells and the fluid are discharged.
1. A mechanical cell lysis apparatus comprising:
an inlet port through which fluid is supplied, wherein the fluid contains cells;
an outlet port through which disrupted cells and the fluid are discharged;
a fluid channel defining a passageway between the inlet port and the outlet port through which the fluid supplied from the inlet port flows, wherein the fluid channel has a first end and a second end, wherein the first end is enlarged from one end of the inlet port, the first end forming an acute angle, and the second end is narrowed at a proximal end of the outlet port;
a flow distribution structure within the fluid channel allowing uniform distribution of the fluid within the fluid channel;
a micro-filter array to remove foreign matter from the fluid, wherein the micro-filter array is downstream of the flow distribution structure; and
a nano blade array including a plurality of nano blades having pointed leading ends and arranged such that the pointed leading ends of the nano blades are oriented in a direction of the fluid supplied through the inlet port to disrupt the cells passing therethrough, wherein the nano blade array is downstream of the micro-filter array.
2. The apparatus according to claim 1 , wherein the plurality of nano blades in the nano blade array is disposed parallel to flow of the fluid supplied through the inlet port.
3. The apparatus according to claim 1 , wherein the nano blade array is disposed across the fluid channel connecting the inlet port to the outlet port.
4. The apparatus according to claim 1 , wherein the micro-filter array comprises at least two rows of micro-filters, one row of micro-filters near the inlet port having a greater distance between micro-filters than the other row of micro-filters.
5. The apparatus according to claim 1 , wherein the inlet port, the nano blade array, the fluid channel and the outlet port are formed on a main panel, and a cover panel is attached to an upper side of the main panel.
6. The apparatus according to claim 5 , wherein the inlet port and the outlet port are placed coplanar with the fluid channel connecting the inlet port to the outlet port.
7. The apparatus according to claim 5 , wherein the cover panel is provided with an inlet tube connected to the inlet port and an outlet tube connected to the outlet port.
8. The apparatus according to claim 5 , wherein the inlet port is configured to be attached to a connector connected to an external device.
9. The apparatus according to claim 8 , wherein the external device comprises a syringe.
10. The apparatus according to claim 1 , wherein the inlet port, the fluid channel, the nano blade array, and the outlet port are formed on a main panel through crystalline wet etching.
11. The apparatus according to claim 1 , wherein the inlet port, the fluid channel, the nano blade array, and the outlet port are formed by injection using stampers corresponding thereto.