Method of enriching for pancreatic endoderm cells
The present disclosure relates to compositions and methods comprising cell surface markers for hES-derived cells, in particular, endoderm lineage cells including pancreatic endoderm-type cells, derived from hES cells.
1. A method of enriching for pancreatic endoderm cells comprising:
a) exposing an in vitro cell population comprising pancreatic endoderm cells to a ligand that binds CD142, CD55, CD57, or CD98; and
b) selecting cells in the in vitro cell population that express CD142 or CD57, or selecting cells in the in vitro cell population that do not express CD55 or CD98, thereby enriching for pancreatic endoderm cells.
2. The method of claim 1 , wherein the pancreatic endoderm cells are derived from in vitro cultured pluripotent cells.
3. The method of claim 1 , wherein the cells selected express CD142.
4. The method of claim 1 , wherein the cells selected express CD57.
5. The method of claim 1 , wherein the cells selected express CD55.
6. The method of claim 1 , wherein the cells selected express CD98.
7. The method of claim 1 , wherein the cells selected express CD142 but do not express CD55.
8. The method of claim 1 , wherein the cells selected express CD142 but do not express CD98.
9. The method of claim 1 , wherein greater than 50% of the pancreatic endoderm cells selected in step b) express CD142 and pancreatic and duodenal homeobox gene 1 (PDX1).
10. The method of claim 1 , wherein greater than 50% of the pancreatic endoderm cells selected in step b) express CD57 and PDX1.
11. The method of claim 1 , wherein the ligand comprises an antibody or binding fragment thereof.
12. The method of claim 1 , wherein the ligand comprises a detectable label.
13. The method of claim 1 , wherein the ligand comprises a magnetic particle.
14. The method of claim 1 , wherein the cells are selected by Fluorescence Activated Cell Sorting (FACS).