Collagen-binding synthetic peptidoglycans, preparation, and methods of use
This invention relates to collagen-binding synthetic peptidoglycans and engineered collagen matrices comprising a collagen matrix and a collagen-binding synthetic peptidoglycan where the collagen-binding synthetic peptidoglycan can be aberrant or can have amino acid homology with a portion of the amino acid sequence of a protein or a proteoglycan that regulates collagen fibrillogenesis. The invention also relates to kits, compounds, compositions, and engineered graft constructs comprising such collagen-binding synthetic peptidoglycans or engineered collagen matrices and methods for their preparation and use.
1. A synthetic peptidoglycan comprising a glycan and from 1 to 50 collagen binding peptides, each of which is covalently bonded to the glycan, wherein the peptides comprise amino acids 1-18 (RRANAALKAGELYKSILY) of SEQ ID NO: 1.
2. The peptidoglycan of claim 1 , wherein the peptides comprise SEQ ID NO: 1.
3. The peptidoglycan of claim 1 , wherein the peptides are bonded to the glycan via a linker having a molecular weight of about 20 to about 500 Daltons.
4. The peptidoglycan of claim 1 , wherein the glycan is a glycosaminoglycan.
5. The peptidoglycan of claim 1 , wherein the glycan is alginate, agarose, chondroitin, dermatan, dermatan sulfate, heparan, heparin, keratin, or hyaluronan.
6. The peptidoglycan of claim 1 , wherein the glycan is dermatan sulfate.
7. The peptidoglycan of claim 1 , wherein the glycan is heparin.
8. The peptidoglycan of claim 1 , wherein the peptidoglycan comprises from 1 to 10 of the peptides.
9. A composition comprising the peptidoglycan of claim 1 and a pharmaceutically acceptable excipient or diluent, or a combination thereof.
10. A synthetic peptidoglycan comprising dermatan sulfate and from 1 to 10 collagen binding peptides, all of which are covalently bonded to the dermatan sulfate, wherein the peptides comprise amino acids 1-18 (RRANAALKAGELYKSILY) of SEQ ID NO: 1.
11. A composition comprising the peptidoglycan of claim 10 and a pharmaceutically acceptable excipient or diluent, or a combination thereof.
12. A synthetic peptidoglycan comprising heparin and from 1 to 10 collagen binding peptides, all of which are covalently bonded to the heparin, wherein the peptides comprise amino acids 1-18 (RRANAALKAGELYKSILY) of SEQ ID NO: 1.
13. A composition comprising the peptidoglycan of claim 12 and a pharmaceutically acceptable excipient or diluent, or a combination thereof.