Oil degumming methods
In alternative embodiments, the invention provides phosphatidylinositol-specific phospholipase C (PI-PLC) enzymes, nucleic acids encoding them, antibodies that bind specifically to them, and methods for making and using them. Industrial methods and products comprising use of these phospholipases are also provided. In certain embodiments, provided herein are methods for hydration of non hydratable phospholipids (NHPs) within a lipid matrix. The methods enable migration of NHPs to an oil-water interface thereby allowing the NHPs to be reacted and/or removed from the lipids. In certain embodiments, provided is a method for removing NHPs, hydratable phospholipids, and lecithins from vegetable oils to produce a degummed oil or fat product that can be used for food production and/or non-food applications. In certain embodiments, provided herein are methods for hydration of NHPs followed by enzymatic treatment and removal of various phospholipids and lecithins. The methods provided herein can be practiced on either crude or water-degummed oils.
1. A method for hydrolyzing, breaking up or disrupting a phospholipid-comprising composition comprising:
(a) providing a phospholipase polypeptide;
(b) providing a composition comprising a phospholipid; and
(c) contacting the polypeptide of step (a) with the composition of step (b) under conditions wherein the phospholipase hydrolyzes, breaks up or disrupts the phospholipid-comprising composition;
wherein the phospholipase polypeptide comprises SEQ ID NO:8.
2. The method of claim 1 , wherein the phospholipase removes or liquefies the phospholipid-comprising composition.
3. The method claim 1 , wherein the polypeptide converts the non-hydratable phospholipid to a hydratable form, wherein the polypeptide catalyzes the hydrolysis of a phospholipid in the composition for a time sufficient to reduce gum mass and increase neutral oils.
4. The method of claim 1 , wherein the polypeptide catalyzes the hydrolysis of a phospholipid in the compostion for a time sufficient to reduce gum mass and increase neutral oils.
5. The method claim 1 , wherein the composition comprises a phospolipid-comprising lipid bilayer or membrane.
6. The method claim 1 , wherein the compostion comprises a plant cell, a bacterial cell, a yeast cell, an insect cell, or an animal cell.