Devices and methods for manipulating components in a fluid sample
Devices for sorting components (e.g., cells) contained in a liquid sample are provided. In certain aspects, the devices include a magnetic separation device and an acoustic concentrator device fluidically coupled to magnetic separation device. Aspects of the invention further include methods for sorting cells in a liquid sample, and systems, and kits for practicing the subject methods.
1. A fluidic device for manipulating components in a liquid sample, the device comprising:
a) a magnetic separator;
b) an acoustic concentrator fluidically coupled to the magnetic separator; and
c) a fluid flow channel between the magnetic separator and the acoustic concentrator, wherein the magnetic separator generates a magnetic field that is spatially separated from the acoustic field generated by the acoustic concentrator.
2. The device according to claim 1 , wherein the acoustic concentrator is fluidically coupled to an output of the magnetic separator.
3. The device according to claim 1 , wherein the liquid sample has a viscosity of about 0.2 cP to about 1.5 cP.
4. The device according to claim 1 , wherein the liquid sample comprises cells.
5. The device according to claim 1 , wherein the device has an output flow rate of about 30 to 100 μL/min.
6. The device according to claim 1 , wherein the magnetic separator comprises a permanent magnet.
7. The device according to claim 1 , wherein the magnetic separator comprises a magnetic field guide.
8. The device according to claim 1 , wherein the acoustic concentrator comprises a flow channel comprising an inlet and an outlet, the inlet and outlet defining a fluid flow path therebetween; and
a vibration generator positioned adjacent to the fluid flow path and configured to produce an acoustic field in the fluid flow path.
9. The device according to claim 8 , wherein the vibration generator is a piezoelectric transducer.
10. The device according to claim 9 , wherein the vibration generator comprises lead zirconate titanate.
11. The device according to claim 1 , comprising a processor configured to apply a magnetic field using the magnetic separator.
12. The device according to claim 1 , comprising a processor configured to control the acoustic concentrator.
13. A flow cytometric system comprising:
i) a flow cytometric sample fluidic subsystem, the flow cytometric sample fluidic subsystem comprising: a) a magnetic separator; b) an acoustic concentrator fluidically coupled to the magnetic separator; and c) a fluid flow channel between the magnetic separator and the acoustic concentrator, wherein the magnetic separator generates a magnetic field that is spatially separated from the acoustic field generated by the acoustic concentrator; and ii) a flow cytometer fluidically coupled to the output of the flow cytometric sample fluidic subsystem.