Peptide conjugated particles
The present invention provides compositions comprising peptide-coupled biodegradable poly(lactide-co-glycolide) (PLG) particles In particular, PLG particles are surface-functionalized to allow for coupling of peptide molecules to the surface of the particles (e.g., for use in eliciting induction of immunological tolerance).
1. A composition comprising surface functionalized biodegradable poly(lactide-co-glycolide) (PLG) particles comprising encapsulated desmoglein 1 and/or desmoglein 3 polypeptides or one or more epitopes thereof, wherein the particle has a negative zeta potential.
2. The composition of claim 1 wherein the desmoglein 1 and/or desmoglein 3 is desmoglein 3 (DSG3).
3. The composition of claim 1 , wherein the particles comprise PLG with a copolymer ratio of about 50:50 of polylactic acid:polyglycolic acid.
4. The composition of claim 1 , wherein the surface functionalization is carboxylation.
5. The composition of claim 4 , wherein the carboxylation is achieved by using poly(ethylene-maleic anhydride) (PEMA).
6. The composition of claim 1 , wherein the particles have a zeta potential of about −100 mV to about −50 mV.
7. The composition of claim 1 , wherein the particles have a zeta potential of about −75 mV to about −50 mV.
8. The composition of claim 1 , wherein the particle has a diameter of between about 200 nm to about 2000 nm.
9. A composition comprising surface functionalized biodegradable PLG particles comprising encapsulated desmoglein 3 polypeptides or one or more epitopes thereof wherein said PLG particles have a copolymer ratio of about 50:50 poly(lactide-co-glycolide), a diameter of about 200 nm to about 2000 nm and a zeta potential of about −50 mv to about −75 mv.
10. A lyophilized composition comprising surface functionalized biodegradable poly(lactide-co-glycolide) (PLG) particles comprising encapsulated desmoglein 1 and/or desmoglein 3 polypeptides or one or more epitopes thereof, herein the particle has a negative zeta potential.
11. The lyophilized composition of claim 10 wherein the desmoglein is desmoglein 3.
12. The lyophilized composition of claim 10 , wherein the particles comprise PLG with a copolymer ratio of about 50:50 of polylactic acid:polyglycolic acid.
13. The lyophilized composition of claim 10 , wherein the surface functionalization is carboxylation.
14. The lyophilized composition of claim 13 wherein the carboxylation is achieved by using poly(ethylene-maleic anhydride) (PEMA).
15. The lyophilize composition of claim 10 , wherein the particles have a zeta potential of about −100 mv to about −50 mV.
16. The lyophilized composition of claim 10 , wherein the particle has a diameter of between about 200 nm to about 2000 nm.
17. A lyophilized composition comprising surface functionalized biodegradable poly(lactide-co-glycolide) (PLG) particles comprising encapsulated desmoglein 3 polypeptide or one or more epitopes thereof wherein said PLG particles have a copolymer ratio of about 50:50 poly(lactide-co-glycolide), a diameter of about 200 nm to about 2000 nm and a zeta potential of about −50 mv to about −100 mv.
18. The composition of claim 1 , wherein said particles encapsulate a desmoglein 3 polypeptide.
19. The composition of claim 1 , wherein said particles encapsulate a desmoglein 1 polypeptide.