Modular platform for multi-tissue integrated cell culture
The systems and methods disclosed herein are generally related to a cell culture system. More particularly, the systems and methods enable the culturing and interconnecting of a plurality of tissue types in a biomimetic environment. By culturing organ specific tissue types within a biomimetic environment and interconnecting each of the organ systems in a physiologically meaningful way, experiments can be conducted on in vitro cells that substantially mimic the responses of in vivo cell populations. In some implementations, the system is used to monitor how organ systems respond to agents such as toxins or medications. The system enables the precise and controlled delivery of these agents, which, in some implementations, enables the biomimetic dosing of drugs in humans to be mimicked.
1. A modular device for culturing cells, the device comprising:
a control plate,
a first cell culture vessel configured to culture a first type of cells;
a second cell culture vessel configured differently than the first cell culture vessel and configured to culture a second type of cells;
a fluid routing plate configured i) to reversibly receive the first and second cell culture vessels such that the first and second cell culture vessels are separately removable from the fluid routing plate, and ii) to couple to the control plate, wherein the fluid routing plate defines a set of channels therethrough to route a fluid flow between the first cell culture vessel and the second cell culture vessel; and
a plurality of actuators within the control plate to selectively block a passage of a fluid through a first fluid flow circuit comprising a first portion of the set of channels between the first and second cell culture vessels and selectively enable the passage of the fluid through a second fluid flow circuit comprising a second portion of the set of channels between the first and second cell culture vessels.
2. The device of 1 , wherein the control plate defines a second set of channels therethrough to route the fluid flow to at least one of the first cell culture vessel and the second cell culture vessel.
3. The device of claim 1 , wherein the first and second cell culture vessels each further comprise a fluid inlet port and a fluid outlet port configured to reversibly and fluidically couple the first and second cell culture vessels to respective channels in the set of channels.
4. The device of claim 1 , wherein the plurality of actuators are configured to control the state of valves within the fluid routing plate to control flow rates of the fluid flow through the fluid routing plate.
5. The device of claim 4 , wherein the plurality of actuators are configured to close the valves by deforming a control membrane within the valves, such that the membrane substantially impedes the flow of fluid through a channel in the fluid routing plate.
6. The device of claim 1 , wherein the plurality of actuators are configured to introduce a predetermined amount of an agent into the fluid flow between the first and second cell culture vessels.
7. The device of claim 6 , wherein the agent is one of a medication and a toxin.
8. The device of claim 1 , wherein a plurality of actuators are configured to withdraw fluid samples from one of the first and second cell culture vessels.
9. The device of claim 1 , wherein the first type of cells corresponds to a portion of a first organ system and the second type of cells corresponds to a portion of a second organ system.
10. The device of claim 9 , wherein the first organ system is different than the second organ system and each of the first organ system and the second organ system correspond to one of a kidney, a lung, and a liver.
11. The device of claim 1 , wherein each of the plurality of actuators is one of a pneumatic actuator, an electro-mechanical actuator, and a valve.
12. The device of claim 1 , wherein the control plate further comprises at least one sensor to measure a parameter in at least one of the first and second cell culture vessels.
13. The device of claim 1 , wherein the first and second cell culture vessels are disposable.
14. The device of claim 1 , wherein the control plate is configured to withstand a sterilization process.
15. The device of claim 1 , wherein first cell culture vessel further comprises a first membrane to support the first type of cells and the second cell culture vessels comprises a second membrane to support the second type of cells.