Immobilized protein system for rapid and enhanced multiplexed diagnostics
The disclosure relates to methods of detecting a neural injury biomarker in a biological sample. The method includes subjecting a biological sample to an assay disclosed that produces a measurable signal and detecting the measurable signal. The presence or absence of the measurable signal indicates the presence or absence of the neural injury biomarker in the sample, and thereby diagnosing a subject as having a neural injury. The disclosure further relates to methods of determining the state of a subject's neural injury. Further disclosed are systems and devices useful in carrying out the methods disclosed.
1. A system comprising:
a platform comprising one or more channels, at least one channel of said one or more channels comprising:
biological assay components suitable to detect the presence of a neural injury biomarker in a biological sample, wherein the neural injury biomarker is an enzyme selected from the group consisting of neuron specific enolase, phosphoglyceromutase, and nucleoside diphosphate kinase A, said components comprising: (i) one or more substrates of the neural injury biomarker and one or more co-factors; and (ii) one or more enzymes capable of acting upon the product of a reaction between the neural injury biomarker and the one or more substrates, wherein at least one enzyme is immobilized within the at least one channel on one or more supports and at least one enzyme is a signal-transducing molecule, said components positioned in the channel to permit a sequential reaction, whereby the neural injury biomarker, if present in the biological sample, will react with the one or more substrates causing a single reaction or a series of coupled reactions that cause production of a measurable signal by the signal-transducing molecule, wherein the measurable signal is selected from the group consisting of a photometrically detectable signal, an electrochemically detectable signal, a colorimetrically detectable signal, and a fluorescent signal.
2. The system according to claim 1 further comprising:
at least one of the one or more channels that serves as a negative control.
3. The system according to claim 1 further comprising:
at least one of the one or more channels that serves as a positive control.
4. The system according to claim 1 further comprising:
a measurable signal detector.
5. The system according to claim 1 ,
wherein the one or more supports comprises a nanoparticle.
6. The system according to claim 1 , wherein the neural injury biomarker is neuron specific enolase.
7. The system according to claim 1 , wherein the neural injury biomarker is phosphoglyceromutase.
8. The system according to claim 1 , wherein the neural injury biomarker is nucleoside diphosphate kinase A.
9. The system according to claim 1 , wherein the measurable signal is photometrically detectable.
10. The system according to claim 1 , wherein, the measurable signal is electrochemically detectable.
11. The system according to claim 1 , wherein, the measurable signal is colorimetrically detectable.
12. The system according to claim 1 , wherein, the measurable signal is fluorescent.