Sample processing system
In accordance with an embodiment of a system for handling and processing chemical and/or biological samples, a MicroChamber comprises a substrate, a reservoir formed on the substrate for receiving a chemical and/or biological sample, and an encoder such as a barcode or other suitable device. The encoder encodes information describing at least one characteristic of the substrate and/or reservoir.
1. A sample processing system comprising:
an automated controller;
a dispenser, wherein the automated controller is programmed to operate the dispenser to create a microchamber on a substrate by dispensing a substance on the substrate to create a barrier around at least a portion of a sample on the substrate; and
a cover handling device, wherein the automated controller is programmed to operate the cover handling device to automate placement and removal of a cover on the microchamber; and
a sealing assembly comprising a sealant pen and a sealant valve controller, wherein the automated controller is programmed to operate the sealing assembly such that the sealant pen dispenses sealant and the sealant valve controller is adapted to control the rate and/or volume of sealant that is dispensed; and
a plurality of temperature control assemblies that are independently controllable to actively heat and actively cool each of a plurality of substrates independently of one another,
wherein the microchamber comprises a reservoir formed on the substrate comprising at least a portion of the sample, a cover and a vesicle coupled to the cover and containing a substance to be dispensed in the reservoir.
2. The sample processing system according to claim 1 further comprising:
a reagent dispensing device configured to apply one or more reagents to the sample.
3. The sample processing system according to claim 1 further comprising:
a cover dispenser that individually dispenses covers;
a robotic head adapted to move relative to a cover dispenser and the substrate; and
an effector coupled to the robotic head that grips and releases the covers, the controller is configured to operate the robotic head and the effector to remove the cover from the cover dispenser, move the cover to a specified position, place the cover over the microchamber, and remove the cover from the microchamber.
4. The sample processing system according to claim 3 wherein:
the effector further comprises at least one of the group consisting of: a vacuum pad, an electromagnetic attachment device, and a mechanical robotic attachment device, that grips and releases the covers.
5. The sample processing system according to claim 3 wherein:
the robotic head further comprises at least one of the group consisting of:
a closed loop motion controller and an open loop motion controller.
6. The sample processing system according to claim 3 further comprising:
a plurality of cover dispensers configured to dispense covers of different sizes.
7. The sample processing system according to claim 3 further comprising:
a program code executable by the controller and configured to:
control automatic placement of the cover in a phased manner to facilitate escape of captive air bubbles from the microchamber and enclose fluid in the microchamber.
8. The sample processing system according to claim 3 further comprising:
the controller that operates the robotic head and effector to remove the cover in a manner that avoids damaging a sample on the substrate.
9. The sample processing system according to claim 3 further comprising:
the sealing assembly that further comprises a sealant reservoir coupled to the sealant pen, and the sealant pen valve that is manipulated by the robotic head and operated by the controller to selectively eject a pattern of sealant to seal the microchamber.
10. The sample processing system according to claim 1 further comprising:
the controller is configured to operate the dispenser to dispense a fluid micro-volume on the substrate within the microchamber.
11. The sample processing system according to claim 9 further comprising:
the controller is configured to control automatic heating of the substrate while the sealant substantially prevents fluid evaporation from the sealed microchamber.
12. The sample processing system according to claim 3 further comprising:
a plurality of covers including a pattern printed on one side to prevent sticking of adjacent covers in the cover dispenser.
13. The sample processing system according to claim 12 further comprising:
a plurality of substrates printed with a TEFLON™ pattern in a configuration that reduces incidence and magnitude of air bubbles trapped under the cover.
14. The sample processing system according to claim 1 further comprising:
a pipette tip handling device operated by the controller to handle a plurality of different sized pipette tips;
a wash head operated by the controller to deliver a plurality of selected bulk solutions in a plurality of controlled volumes to the substrate; and
a blow head operated by the controller to dry the substrate.
15. The sample processing system according to claim 1 further comprising:
the sealant pen operated by the controller to dispense a selected amount of sealant in a programmed pattern on the substrates, the sealant being applied to seal a cover to the substrate.
16. The sample processing system according to claim 3 further comprising:
a stationary platform configured to hold a plurality of substrates; and
the robotic head is operated by the controller to process samples on the substrates.
17. The sample processing system according to claim 2 further comprising:
the controller operates the reagent dispensing device and the cover handling device to dispense a fluid micro-volume on the sample and cover the fluid micro-volume with a cover during at least one of the group comprising: chemical, biological, genomics, proteomics, histology, and cytology assays.
18. The sample processing system according to claim 17 further comprising:
a controller coupled to the temperature control assemblies that operates the temperature control assemblies to automate variable temperature processing of samples on the substrates including controlled temperature processing of at least one of the group consisting of: DNA chips, protein chips, tissues, tissue micro-assays, chemical assays, biochemical assays, and biological assays, as well as Hybridization, Fluorescence In Situ Hybridization (FISH), In Situ Hybridization (ISH) assays, and assays involving a ligand and molecular target.
19. The sample processing system according to claim 1 , wherein the substrate is a glass slide.