Use of vitelline protein B as a microencapsulating additive
The present invention includes compositions and methods for the use of an encapsulation additive having between about 0.1 to about 30 percent isolated and purified vitelline protein B to provide for mixed and extended release formulations.
1. A method of making an extended release formulation comprising:
providing a recombinant scrambled vitelline protein B (vpB) peptide which retains the same amino acid ratio as the ratio of amino acids 82-187 of the amino acid sequence selected from the group consisting of SEQ ID NO:1 and SEQ ID NO:2;
combining the recombinant scrambled peptide, one or more active agents, a crosslinking agent and an alginate; and
crosslinking the recombinant scrambled peptide, the one or more active agents, the crosslinking agent and the alginate to form the extended release formulation.
2. The method of claim 1 , wherein the one or more active agents are released for between about 1 hour to about 8 hours, or between about 1 hour to about 2 weeks, or between about 1 hour to about 6 months.
3. The method of claim 1 , wherein the one or more active agents comprise a pharmaceutical agent, an enzyme, a cytokine, a growth promoting agent, an antibody, an antigen, a vaccine, a cell, a live-attenuated pathogen, a heat-killed pathogen, a virus, a bacteria, a fungi, a peptide, a protein, a carbohydrate, a nucleic acid, a lipid, mixtures and combinations thereof.
4. The method of claim 1 , wherein the one or more active agents, and the alginate are combined with the cross-linking agent and the peptide by coacervation, spray freezing, extrusion, nebulization, spray drying or into liposomes.
5. The method of claim 1 , wherein the crosslinking agent is poly-L Lysine and the ratio of the poly-L lysine to the peptide is between 30:70 to 70:30 weight to weight.
6. The method of claim 1 , wherein the crosslinking agent is poly-L Lysine and the ratio of the poly-L lysine to the peptide is between about 50:50 weight to weight.
7. The method of claim 1 , wherein the crosslinking agent is a cation or a polycation.
8. The method of claim 1 , wherein the one or more active agents is an antigen and the antigen comprises Brucella sp.
9. The method of claim 1 , wherein the method further comprises the step of lyophilizing the composition.
10. A method of making an extended release formulation comprising:
providing a recombinant scrambled vitelline protein B (vpB) peptide which retains the same amino acid ratio as the ratio of amino acids in a central 33% portion of the amino acid sequence selected from the group consisting of SEQ ID NO:1 and SEQ ID NO:2;
combining the recombinant scrambled peptide, a poly-Lysine crosslinking agent, one or more active agents and an alginate to form a release formulation, wherein the ratio of the peptide to the poly-Lysine crosslinking agent is between 30:70 to 70:30 weight to weight; and
crosslinking the recombinant scrambled peptide, the poly-Lysine crosslinking agent, the one or more active agents and the alginate to form the extended release formulation.
11. The method of claim 10 , wherein the crosslinking agent is poly-L Lysine.