Spinocerebellar ataxia type 8 and methods of detection
The present invention provides an isolated nucleic acid molecule containing a repeat region of an isolated spinocerebellar ataxia type 8 (SCA8) coding sequence, the coding sequence located within the long arm of chromosome 13, and the complement of the nucleic acid molecule. Diagnostic methods based on identification of this repeat region are also provided.
1. A method for sequencing a SCA8 coding sequence in a human subject, comprising:
(a) providing a first reaction mixture comprising a DNA molecule in a biological specimen from a human subject and a first primer pair, wherein the DNA molecule comprises a SCA8 coding sequence, and wherein the primers in the first primer pair hybridize to a nucleotide sequence selected from the group consisting of nucleotides 1-448 and nucleotides 726-1159 of SEQ ID NO:1 or complements thereof, nucleotides 1-1070 and nucleotides 1350-1472 of SEQ ID NO:2 or complements thereof, and nucleotides 1-636 and nucleotides 915-1037 of SEQ NO:3 or complements thereof;
(b) amplifying the SCA8 coding sequence from the human subject to produce a first amplified DNA fragment;
(c) providing a second reaction mixture comprising the first amplified DNA and a second primer pair;
(d) amplifying the SCAB coding sequence to produce a second amplified DNA, fragment; and
(e) sequencing the second amplified DNA fragment.
2. The method according to claim 1 , wherein a first primer of the first primer pair is selected from nucleotides 1-448 of SEQ ID NO:1 and a second primer of the first primer pair is selected from nucleotides complementary to nucleotides 726-1159 of SEQ ID NO:1, wherein each of the first and second primers comprises at least 11 nucleotides.
3. The method according to claim 2 , wherein the first primer is selected from the group consisting of SEQ ID NO:5, SEQ ID NO:8, and SEQ ID NO:4, and wherein the second primer is selected from the group consisting of SEQ ID NO:6, SEQ ID NO:9, and SEQ ID NO:12.
4. The method of claim 1 , wherein the second primer pair comprises a first primer selected from nucleotides 449-725 of SEQ ID NO:1 and a second primer selected from nucleotides 726-1, 159 of SEQ ID NO:1, wherein each of the first and second primers comprises at least 11 nucleotides.
5. The method according to claim 1 , wherein the repeat region comprises at least about 80 CTG repeats.
6. The method according to claim 1 , wherein the repeat region comprises at least about 92 CTG/CTA repeats.
7. A method for sequencing a SCA8 coding sequence from a human subject, comprising:
providing a reaction mixture comprising a DNA molecule in a biological specimen from a human subject and a primer pair, wherein:
the DNA molecule comprises a SCA8 coding sequence, and
the primers in the primer pair hybridize to a nucleotide sequence selected from the group consisting of nucleotides 1-448 and nucleotides 726-1159 of SEQ ID NO:1 or complements thereof, nucleotides 1-1070 and nucleotides 1350-1472 of SEQ ID NO:2 or complements thereof, and nucleotides 1-636 and nucleotides 915-1037 of SEQ ID NO:3 or complements thereof;
amplifying the SCA8 coding sequence from the human subject to produce an amplified DNA fragment; and
sequencing the amplified DNA fragment.
8. The method according to claim 7 , wherein a first primer of the primer pair is selected from nucleotides 1-448 of SEQ ID NO:1 and a second primer of the primer pair is selected from nucleotides complementary to nucleotides 726-1159 of SEQ ID NO:1, wherein each of the first and second primers comprises at least 11 nucleotides.
9. The method according to claim 8 , wherein the first primer is selected from the group consisting of SEQ ID NO:5, SEQ ID NO:8, and SEQ ID NO:4, and wherein the second primer is selected from the group consisting of SEQ ID NO:6, SEQ ID NO:9, and SEQ ID NO:12.
10. The method according to claim 7 , wherein the repeat region comprises at least about 80 CTG repeats.
11. The method according to claim 7 , wherein the repeat region comprises at least about 92 CTG/CTA repeats.