Retinal OFF circuit-specific promoter
View Patent ↗The present invention provides an isolated nucleic acid molecule comprising, or consisting of, the nucleic acid sequence of SEQ ID NO:1 or a nucleic acid sequence of at least 200 bp having at least 70% identity to said sequence of SEQ ID NO:1, wherein said isolated nucleic acid molecule specifically leads to the expression in cones and/or OFF bipolar cells of a gene when operatively linked to a nucleic acid sequence coding for said gene.
1. An isolated nucleic acid molecule comprising the nucleic acid sequence of SEQ ID NO: 1, wherein said isolated nucleic acid molecule specifically directs expression of a gene in at least one cell selected from cones and OFF bipolar cells, when a nucleic acid sequence comprising the gene is operatively linked to said isolated nucleic acid molecule.
2. An expression cassette comprising, the isolated nucleic acid according to claim 1 .
3. A vector comprising the expression cassette of claim 2 .
4. The vector of claim 3 , wherein said vector is a viral vector.
5. An isolated cell comprising the expression cassette of claim 2 .
6. The cell of claim 5 wherein the expression cassette is stably integrated into the genome of said cell.
7. The isolated nucleic acid molecule of claim 1 , wherein the gene encodes a photoreceptor.
8. A kit for expressing a gene in the at least one cell selected from cones and OFF bipolar cells, wherein the kit comprises the isolated nucleic acid molecule according to claim 1 .
9. A method of expressing a gene specifically in the at least one cell selected from cones and OFF bipolar cells, wherein the method comprises transfecting the cell with a vector according to claim 3 .