Burdock fruit extract containing arctigenin at high content and process for producing same
PROBLEM TO BE SOLVED A burdock fruit extract containing arctigenin at high content and its production method are provided, and both of which are used for treatment of pancreatic cancer. SOLUTION The burdock fruit extract containing arctigenin at high content by enzymatically converted arctiin into arctigenin with beta-glucosidase, which is an enzyme occurring endogenously in a burdock fruit, and adding ethanol, extracting, concentrating and then freeze-drying or spray drying.
1. A method for producing an extract from burdock fruit, said extract containing arctigenin at a high content of more than 3% of arctigenin, comprising the steps of:
a) cutting a burdock fruit;
b) adding water to said burdock fruit;
c) agitating said burdock fruit in water at a temperature of 20-60° C., thereby converting arctiin in said burdock fruit into arctigenin by enzymatic conversion using beta-glucosidase, wherein said beta-glucosidase consists of endogenous beta-glucosidase that is inherently present in said burdock fruit used in herbal medicine;
d) adding ethanol to the burdock fruit in water after the agitating step c) to form a solution comprising a burdock fruit extract;
e) obtaining the burdock fruit extract from the solution; and
f) concentrating and freeze-drying or spray drying the burdock fruit extract after the obtaining step e) to produce an extract from burdock fruit containing arctigenin at a high content of more than 3% of arctigenin.
2. The method for producing burdock fruit extract containing arctigenin at high content as claimed in claim 1 further comprising, in the step f), adding dextrin to the burdock fruit extract after concentrating and spray drying after the extraction the burdock fruit extract.