Method for producing cell culture vessel
This invention provides a cost-effective technique for mass-production of a cell culture vessel that allows mass cell culture to be performed in a manner that allows cells to be stably detached from the culture vessel. Specifically, the invention provides a method for producing a cell culture vessel suitable for large-capacity culture comprising cutting a long-sized cell support film to obtain a sheet-like cell support film, fixing the film to a first member, which is not closed, and bonding other members thereto. Thus, a cell culture vessel comprising a container section in which a cell support film is fixed to an inner wall surface can be produced.
1. A method for producing a cell culture vessel comprising a container section in which an inner chamber for accommodating cells and media is provided, the container section comprising a cell support film, which is at least provided with a film substrate layer and, disposed thereon, a stimuli-responsive polymer layer having a cell-adhesive surface capable of changing into a non-cell-adhesive surface upon reception of a particular stimulus, fixed to at least a part of the inner wall surface facing the inner chamber in a manner such that the stimuli-responsive polymer layer is disposed facing the inner chamber, the method comprising:
a step of cutting a long-sized cell support film to prepare a sheet-like cell support film;
a step of cell support film fixation comprising fixing a sheet-like cell support film to a first member of the container section having a region of an opened inner wall surface to which the cell support film is fixed, thereby producing a film-fixed first member;
a step of member bonding for constituting the container section by bonding the film-fixed first member to one or more other members; and
a step of sterilization by irradiation of the cell culture vessel with a γ beam under vacuum conditions.
2. The method according to claim 1 , further comprising
a step of producing a long-sized cell support film, wherein the step of producing a long-sized cell support film comprises unwinding the rolled long-sized film substrate to feed the unwound film substrate, forming a stimuli-responsive polymer layer on the unwound film substrate, and rewinding the formed film substrate as a roll.
3. The method according to claim 1 , wherein the step of cutting comprises cutting the long-sized cell support film to prepare a polygonal sheet-like cell support film.
4. The method according to claim 1 , wherein the step of cell support film fixation comprises a step of allowing the sheet-like cell support film to adhere to the first member with the aid of an adhesive.
5. The method according to claim 1 , wherein the step of cell support film fixation comprises a step of fixing the sheet-like cell support film to the first member by heat sealing.
6. The method according to claim 1 , wherein the step of cell support film fixation comprises a step of fixing the sheet-like cell support film to the first member by ultrasonic welding.
7. The method according to claim 1 , wherein the step of cell support film fixation comprises a step of fixing the sheet-like cell support film to the first member by laser-beam welding.
8. The method according to claim 1 , wherein the step of cell support film fixation comprises a step of introducing resin into an injection mold in which the sheet-like cell support film is disposed in advance to prepare the first member and obtaining the film-fixed first member.
9. The method according to claim 1 , wherein the step of cell support film fixation comprises a step of introducing resin into an injection mold in which the sheet-like cell support film is disposed in advance to prepare the first member and obtaining the film-fixed first member, the cell support film further comprising a heat-sealable resin layer formed on a surface of the film substrate layer opposite from the stimuli-responsive polymer layer.
10. The method according to claim 1 , wherein the step of cell support film fixation comprises a step of fixing the sheet-like cell support film to the first member with the use of a physical locking means.