Immunopotentiator-linked oligomeric influenza immunogenic compositions
Disclosed herein are immunogenic compositions for preventing infection with influenza viruses wherein the immunogenic compositions comprises an immunogen such as a hemagglutinin of an influenza virus, and an immunopotentiator such as an Fc fragment of human IgG and optionally a stabilization sequence. The immunogen is linked to the stabilization sequence which in turn is linked to the immunopotentiator.
1. An immunogenic composition for induction of an immune response against influenza virus comprising a fusion polypeptide comprising:
an immunogen sequence from an influenza virus;
an immunopotentiator sequence comprising the Fc fragment of human IgG, or a muramyl peptide;
and a stabilization sequence, wherein said stabilization sequence is foldon.
2. The immunogenic composition of claim 1 , wherein said immunogen sequence is a hemagglutinin sequence of said influenza virus.
3. The immunogenic composition of claim 1 , wherein said immunogen sequence is a neuraminidase sequence of an influenza virus.
4. The immunogenic composition of claim 1 , wherein said immunogen sequence is a membrane protein sequence of an influenza virus.
5. The immunogenic composition of claim 1 , wherein said fusion polypeptide is produced in a mammalian expression system.
6. The immunogenic composition of claim 2 , wherein said hemagglutinin sequence is a fragment of a hemagglutinin sequence selected from the group consisting of HA1 HA2 and RBD.
7. The immunogenic composition of claim 1 , wherein said fusion polypeptide is selected from the group consisting of HA1-Fd-hFc, HA-Fd-hFc, HA2-Fd-hFc, HA-RBD-Fd-hFc, and HA1+3-259-Fd-hFc.
8. The immunogenic composition of claim 1 , wherein said immunogenic composition further comprises an adjuvant.