Microbial nanowires and methods of making and using
Electrically conductive nanowires, and genetically or chemically modified production and use of such nanowires with altered conductive, adhesive, coupling or other properties are described. The disclosed nanowires are used as device or device components or may be adapted for soluble metal remediation.
1. An isolated or recombinant Geobacteraceae nanowire peptide comprising a truncated nanowire peptide fused to a fusion partner peptide at the N-terminus of the nanowire peptide, wherein the truncation comprises a one to 29 amino acid truncation at the N-terminus or at the C-terminus of a nanowire peptide, and wherein the truncated nanowire peptide assembles into a conductive pilus and is electrically conductive, the nanowire peptide comprising an amino acid sequence selected from SEQ ID NO:1-10 and variants thereof.
2. The nanowire peptide of claim 1 , wherein the peptide is genetically or chemically modified to modulate the conductive, adhesive, assembly or coupling properties of the nanowire peptide.
3. A pilus comprising the nanowire peptide of claim 1 .
4. The nanowire peptide of claim 1 , wherein the nanowire peptide is truncated at the N-terminus.
5. The nanowire peptide of claim 1 , wherein the nanowire peptide is truncated at the N-terminus by 1 to 29 amino acids.
6. The nanowire peptide of claim 1 , wherein the nanowire peptide is truncated at the N-terminus by 10 to 29 amino acids.
7. The nanowire peptide of claim 1 , wherein the amino acid sequence of the nanowire peptide has at least 95% amino acid identity with any of SEQ ID NOS: 1-10.
8. The nanowire peptide of claim 1 , wherein the truncated nanowire peptide comprises an amino acid sequence selected from SEQ ID NOS: 26-29 and variants or combinations thereof.
9. The nanowire peptide of claim 8 , wherein the amino acid sequence of the truncated nanowire peptide has at least 95% amino acid identity to any of SEQ ID NOS: 26-29 or combinations thereof.
10. A method of producing nanowire peptides fused to a fusion partner peptide at the N-terminus of the nanowire peptide comprising:
expressing a fusion protein comprising a fusion partner peptide fused to the N-terminus of a nanowire peptide,
wherein the fusion protein is expressed by a host cell, and the nanowire peptides comprise an isolated or recombinant Geobacteraceae nanowire peptide comprising a truncated nanowire peptide, wherein the truncation comprises a one to 29 amino acid truncation at the N-terminus or at the C-terminus of the nanowire peptide, and wherein the nanowire peptide assembles into a conductive pilus and is electrically conductive, the nanowire peptide comprising an amino acid sequence selected from SEQ ID NO:1-10 and variants thereof.
11. The method of claim 10 wherein the fusion partner peptide comprises a chitin-binding domain.
12. The method of claim 10 further comprising affinity purifying the fusion protein; and removing the fusion partner peptide from the fusion protein to allow the nanowire peptide to assemble.
13. The method of claim 12 , wherein a pilin within each of the one or more conductive pili comprises a truncated nanowire peptide from Geobacteraceae bacterium wherein the truncation comprises a one to 29 amino acid truncation at an N-terminus or at a C-terminus, and wherein the polypeptide assembles into the conductive pilus.
14. The method of claim 12 wherein the conductive pili are expressed in biofilms.
15. An isolated or recombinant Geobacteraceae nanowire peptide comprising a truncated nanowire peptide fused to a fusion partner peptide comprising a chitin-binding domain (CBD) at the N-terminus of the nanowire peptide, wherein the truncation comprises a one to 29 amino acid truncation at the N-terminus or at the C-terminus of a nanowire peptide, and wherein the truncated nanowire peptide assembles into a conductive pilus and is electrically conductive, the nanowire peptide comprising an amino acid sequence selected from SEQ ID NO:1-10 and variants thereof.