Granted Patent
B2
US 9,605,243 · App. 14/066,441 · Granted Mar 28, 2017
Markers of definitive endoderm
Inventors:
Kevin Allen D'Amour (San Diego, CA); Alan D. Agulnick (San Diego, CA); Emmanuel Edward Baetge (Encinitas, CA)
Assignee:
ViaCyte, Inc.
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Abstract
Disclosed herein are reagent-cell complexes comprising one or more definitive endoderm cells. Also described herein are compositions for detecting definitive endoderm. Method of enriching, isolating and/or purifying definitive endoderm cells are also disclosed.
Claims (4)
1. An in vitro method of producing cells which express thyroid transcription factor 1 (TITF1) from mammalian definitive endoderm cells comprising the steps of:
(i) culturing mammalian definitive endoderm cells with an effective amount of Wnt3a for at least 7 days,
(ii) enriching for cells which express TITF1.
2. The in vitro method of claim 1 , wherein step (i) further comprises providing less than 2% serum to the definitive endoderm cells.
Assignments (2)
ASSIGNMENT OF ASSIGNOR'S INTEREST
Recorded Nov 18, 2016
From: D'AMOUR, KEVIN ALLEN; AGULNICK, ALAN D.; BAETGE, EMMANUEL EDWARD
To: CYTHERA, INC.
Reel/Frame 040364/0649 →
ASSIGNMENT OF ASSIGNOR'S INTEREST
Recorded Nov 18, 2016
From: CYTHERA, INC.
To: VIACYTE, INC.
Reel/Frame 040647/0074 →
Continuity (7)
Continuation
12093590
Provisional Application
60736598
· Nov 14, 2005
Provisional Application
60532004
· Dec 23, 2003
Provisional Application
60586566
· Jul 9, 2004
Provisional Application
60587942
· Jul 14, 2004