Process of production and extra-cellular secretion of lipids

1. A process of enhanced production and extra-cellular secretion of lipids comprising:

a. obtaining a biomass of a microalgae, wherein the microalgae are selected from the group consisting of Chlamydomonas reinhardtii, Chlamydomonas caudate Willie, Chlamydonomas moewusii, Chlamydonomas nivalis , and Chlamydonomas ovoidae ; and

b. culturing the biomass of said microalgae in a modified growth medium deficient in nitrogen and iron, wherein the modified growth medium deficient in nitrogen and iron comprises:

i) yeast extract in the range of 0.05%-0.2% (w/v);

ii) beef extract in the range of 0.05%-0.2% (w/v); and

iii) glucose in the range of 0.5%-3.0% (w/v), wherein the production and extra-cellular secretion of lipids by the microalgae biomass cultured in said modified growth medium deficient in nitrogen and iron is enhanced.

2. The process as claimed in claim 1 , wherein the modified growth medium deficient in nitrogen and iron comprises:

a. 0.1% (w/v) yeast extract;

b. 0.1% (w/v) beef extract; and

c. 2.3% (w/v) glucose.

3. The process as claimed in claim 1 , wherein the pH of the modified growth medium deficient in nitrogen and iron is in the range of 6.5-7.5.

4. The process as claimed in claim 1 , wherein the biomass of the microalgae is cultured in said modified growth medium deficient in nitrogen and iron subjected to a light cycle period ranging from 4.69-hours to 24-hours.

5. The process as claimed in claim 4 , wherein the biomass of the microalgae is cultured in said modified growth medium deficient in nitrogen and iron subjected to a light cycle period ranging from 12-hours to 18-hours.

6. The process as claimed in claim 1 , wherein 50%-70% (w/v) of lipids produced is secreted extra-cellularly.

7. The process as claimed in claim 1 , wherein more than 90% (w/v) of the extra-cellularly secreted lipids are triglycerides.