Simian adenovirus 41 and uses thereof
Novel simian adenovirus 41 and two isolates thereof are described. Various uses of these isolates, including construction of a recombinant vector which comprises simian adenovirus 41 sequences and a heterologous gene under the control of regulatory sequences are provided. A cell line which expresses simian adenovirus 41 gene(s) is also disclosed. Methods of using the vectors and cell lines are provided.
1. A vector comprising a nucleic acid sequence encoding a simian adenovirus 41 (SAdV-41) hexon protein with the amino acids 1 to 943 of SEQ ID NO: 11 or the amino acids 1 to 943 of SEQ ID NO: 44; said vector further comprising a heterologous gene operably linked to expression control sequences.
2. A composition comprising the vector according to claim 1 and a pharmaceutically acceptable carrier.
3. The vector according to claim 1 , comprising a nucleic acid sequence encoding a SAdV-41.1 penton protein with the amino acids 1 to 584 of SEQ ID NO: 6 or a SAdV-41.2 penton protein with the amino acids 1 to 584 of SEQ ID NO: 39.
4. The vector according to claim 1 , comprising a nucleic acid sequence encoding a SAdV-41.1 fiber protein with the amino acids 1 to 321 of SEQ ID NO: 21 or a SAdV-41.2 fiber protein with the amino acids 1 to 325 of SEQ ID NO: 54.
5. The vector according to claim 1 , comprising at least one nucleic acid sequence encoding:
a SAdV-41.1 penton protein with the amino acids 1 to 584 of SEQ ID NO: 6 or a SAdV-41.2 penton protein with the amino acids 1 to 584 of SEQ ID NO: 39; and
a SAdV-41.1 fiber protein with the amino acids 1 to 321 of SEQ ID NO: 21 or a SAdV-41.2 fiber protein with the amino acids 1 to 325 of SEQ ID NO: 54.
6. The vector according to claim 5 , comprising at least one nucleic acid sequence encoding
the SAdV-41 hexon protein with the amino acids 1 to 943 of SEQ ID NO: 11 or the amino acids 1 to 943 of SEQ ID NO: 44;
the SAdV-41.1 penton protein with the amino acids 1 to 584 of SEQ ID NO: 6; and
the SAdV-41.1 fiber protein with the amino acids 1 to 321 of SEQ ID NO: 21.
7. The vector according to claim 5 , comprising at least one nucleic acid sequence encoding
the SAdV-41 hexon protein with the amino acids 1 to 943 of SEQ ID NO: 11 or amino acids 1 to 943 of SEQ ID NO: 44;
the SAdV-41.2 penton protein with the amino acids 1 to 584 of SEQ ID NO: 39; and
the SAdV-41.2 fiber protein with the amino acids 1 to 325 of SEQ ID NO: 54.
8. The vector according to claim 1 , comprising at least one nucleic acid sequence encoding one or more simian adenovirus proteins selected from
E1a having the amino acid sequence of SEQ ID NO: 29 or SEQ ID NO: 63;
E1b, small T/19K having the amino acid sequence of SEQ ID NO: 23 or SEQ ID NO: 56;
E1b, large T/55K having the amino acid sequence of SEQ ID NO: 2 or SEQ ID NO: 35;
IX, having the amino acid sequence of SEQ ID NO: 3 or SEQ ID NO: 36;
52/55D, having the amino acid sequence of SEQ ID NO: 4 or SEQ ID NO: 37;
IIIa, having the amino acid sequence of SEQ ID NO: 5 or SEQ ID NO: 38;
Penton, having the amino acid sequence of SEQ ID NO: 6 or SEQ ID NO: 39;
VII, having the amino acid sequence of SEQ ID NO: 7 or SEQ ID NO: 40;
V, having the amino acid sequence of SEQ ID NO: 8 or SEQ ID NO: 41;
pX, having the amino acid sequence of SEQ ID NO:9 or SEQ ID NO: 36;
VI, having the amino acid sequence of SEQ ID NO: 10 or SEQ ID NO: 43;
Endoprotease, having the amino acid sequence of SEQ ID NO: 12 or SEQ ID NO: 58;
100 kD, having the amino acid sequence of SEQ ID NO: 13 or SEQ ID NO: 59;
33 kD homolog, having the amino acid sequence of SEQ ID NO: 31 or SEQ ID NO: 65;
22 kD, having the amino acid sequence of SEQ ID NO: 25 or SEQ ID NO: 45;
VIII, having the amino acid sequence of SEQ ID NO: 14 or SEQ ID NO: 46;
E3/12.5 K, having the amino acid sequence of SEQ ID NO: 15 or SEQ ID NO: 47;
CR1-alpha, having the amino acid sequence of SEQ ID NO: 26 or SEQ ID NO: 60;
gp19K, having the amino acid sequence of SEQ ID NO: 16 or SEQ ID NO: 48;
CR1-beta, having the amino acid sequence of SEQ ID NO: 17 or SEQ ID NO: 49;
CR1-gamma, having the amino acid sequence of SEQ ID NO: 18 or SEQ ID NO: 50;
RID-alpha, having the amino acid sequence of SEQ ID NO: 19 or SEQ ID NO: 52;
RID-beta, having the amino acid sequence of SEQ ID NO: 27 or SEQ ID NO: 61;
E3/14.7K, having the amino acid sequence of SEQ ID NO: 20 or SEQ ID NO: 53; and
Fiber, having the amino acid sequence of SEQ ID NO: 21 or SEQ ID NO: 54.