Efficient process for producing saccharides and ethanol from a biomass feedstock
Described herein is a process for producing saccharides and ethanol from biomass feedstock that includes (a) producing an enzyme composition by culturing a fungal strain(s) in the presence of a lignocellulosic medium, (b) using the enzyme composition to saccharify the biomass feedstock, and (c) fermenting the saccharified biomass feedstock to produce ethanol. The process is scalable and, in certain aspects, is capable of being deployed on farms, thereby allowing local production of saccharides and ethanol and resulting in a reduction of energy and other costs for farm operators. Optional steps to improve the biomass-to-fuel conversion efficiency are also contemplated, as are uses for byproducts of the process described herein.
1. A process for converting a biomass feedstock to a saccharide, the method comprising contacting the biomass feedstock with an enzyme composition, wherein the enzyme composition is produced by culturing Trichoderma SG2, Trichoderma SG4, or a combination thereof, in the presence of a lignocellulosic medium and a starch.
2. The process of claim 1 , wherein when the fungal strain is Trichoderma SG2 and the enzyme composition comprises cellulase, xylanase, beta-glucosidase, beta-xylosidase, amylase, or any combination thereof.
3. The process of claim 1 , wherein the enzyme composition is separated from the lignocellulosic medium by filtration or centrifugation prior to contact with the biomass feedstock.
4. The process of claim 1 , wherein the enzyme composition further comprises a second enzyme composition for hydrolyzing the biomass feedstock, wherein the second enzyme composition is not produced by Trichoderma SG2 or its teleomorph thereof, Trichoderma SG4 or its teleomorph thereof.
5. The process of claim 4 , wherein the second enzyme composition comprises cellulase, xylanase, beta-glucosidase, hemicellulase, or any combination thereof.
6. The process of claim 4 , wherein the second enzyme composition is 10% to 50% by volume of the enzyme composition.
7. The process of claim 4 , wherein the second enzyme composition is 25% to 50% by volume of the enzyme composition.
8. The process of claim 1 , wherein the biomass feedstock comprises (i) an agricultural feedstock, a bioenergy grass, a forestry residue, a logging residue, a sawmill residue, animal manure, a carbohydrate waste, a recycled material, or a combination thereof, and (ii) a starch.
9. The process of claim 8 , wherein the biomass feedstock comprises a corn cob, corn stover, wheat straw, a peanut hull, soy hull, switchgrass, gammagrass, sawdust, paper, a chemically liquefied polymer, a high sugar waste, or any combination thereof.
10. The process of claim 1 , wherein the method is conducted in a bioreactor.
11. The process of claim 1 , wherein the method is conducted in a farm-deployable bioreactor.
12. A process for producing ethanol from biomass feedstock, the method comprising
(a) contacting the biomass feedstock with an enzyme composition to convert the biomass feedstock to a saccharide using the process of claim 1 , wherein the enzyme composition is produced by culturing Trichoderma SG2 , Trichoderma SG4, or a combination thereof, in the presence of a lignocellulosic medium and a starch; and
(b) fermenting the saccharide to produce ethanol.
13. The process of claim 12 , wherein the saccharide is fermented in the presence of yeast.
14. The process of claim 13 , wherein the yeast comprises a Saccharomyces yeast, a Pichia yeast, or a combination thereof.
15. The process of claim 12 , wherein after step (b), the ethanol is isolated by distillation.
16. The process of claim 15 , wherein the ethanol is distilled using an ice-cooled condenser system.
17. The process of claim 12 , wherein the process is a batch process or continuous process.
18. The process of claim 12 , wherein prior to step (a), the biomass feedstock is treated with a dilute acid or dilute base.
19. The process of claim 12 , wherein the process is conducted in the same bioreactor.
20. The process of claim 1 , wherein the enzyme composition further comprises a metal ion selected from the group consisting of barium, cobalt, calcium, iron, potassium, manganese, zinc, or any combination thereof.
21. The process of claim 1 , wherein the starch comprises an expired food product selected from the group consisting of corn starch, potato starch, rice starch, tapioca starch, bread or other bakery products, bread waste, bakery waste, donuts, donut waste, or any combination thereof.
22. The process of claim 1 , wherein prior to contacting the biomass feedstock with the enzyme composition, pretreating the biomass feedstock with an acid, a base, an organic solvent, with heat, or any combination thereof.
23. The process of claim 1 , wherein after converting the biomass feedstock to a saccharide, separating the enzyme composition produced by the process by sand filtration.