Human exhaled aerosol droplet biomarker system and method
A system and method for detecting a biomarker in exhaled breath condensate nanodroplets comprises noninvasively collecting exhaled breath condensate nanodroplets of a subject, and analyzing said nanodroplets utilizing immuno-quantitative polymerase chain reaction to detect one or more target biomarkers.
1. A system for detecting biomarkers in exhaled breath nanodroplets, comprising:
a collection device configured to noninvasively collect exhaled breath condensate nanodroplets from a subject by impacting said nanodroplets into a liquid medium, said collection device comprising an optical particle counter configured to determine number and size distribution of said nanodroplets; and
immuno-quantitative polymerase chain reaction (IqPCR) assay for detecting one or more target biomarkers in said nanodroplets.
2. The system of claim 1 , wherein said IqPCR assay comprises a microbead having an antibody immobilized thereon, wherein said antibody binds to a particular one of said target biomarkers.
3. The system of claim 1 , wherein said IqPCR exhibits at least a 10-fold increase in sensitivity in detection of said target biomarkers as compared to enzyme-linked immuno-sorbent assay (ELISA).
4. The system of claim 3 , wherein said IqPCR exhibits at least a 1000-fold increase in sensitivity in detection of said target biomarkers as compared to enzyme-linked immuno-sorbent assay (ELISA).
5. The system of claim 1 , wherein said biomarkers are associated with a respiratory disease, disorder or infection.
6. The system of claim 1 , wherein said biomarkers comprise cytokines.
7. The system of claim 6 , wherein said biomarkers are selected from the group consisting of IFN-gamma, IL-1 beta, IL-7, IL-8, IL-13, and TNF-alpha.
8. The system of claim 1 , wherein said IqPCR assay comprises a plurality of microbead-antibody complexes for simultaneous detection of multiple target biomarkers.
9. The system of claim 1 , wherein at least one of said target biomarkers is associated with a respiratory disease, disorder or infection.