Cytotoxic T lymphocyte inducing immunogens for prevention treatment and diagnosis of cancer
The present invention relates to compositions and methods for the prevention, treatment, and diagnosis of cancer, especially carcinomas, such as pancreatic carcinoma. The invention discloses peptides, polypeptides, and polynucleotides that can be used to stimulate a CTL response against pancreatic and other cancers.
1. A composition for use in a method of:
(a) eliciting a CTL response against tumor cells presenting an epitopic peptide SEQ ID NO: 04 by administration of a sufficient amount of SEQ ID NO: 04, SEQ ID NO: 46 peptides and a stabilizing agent wherein said peptides are in a stable form in the presence of said stabilizing agent in a subject; or
(b) stimulating an immune response in an immunologically competent animal, said composition consisting of SEQ ID NO: 04 and a stabilizing agent capable of facilitating an immune response thereof.
2. The composition for use in a method of claim 1 , wherein said composition further comprises an adjuvant, optionally wherein said adjuvant is selected from the group consisting of complete Freund's adjuvant, incomplete Freund's adjuvant, Montanide ISA-51, LAG-3, aluminum phosphate, aluminum hydroxide, alum, and saponin.
3. The composition for use in a method of claim 1 , wherein said composition further comprises a cytokine, optionally wherein said cytokine is selected from the group consisting of IL-1, IL-2, IL-7, IL-12, IL-15, TNF, SCF and GM-CSF.
4. The composition for use in a method of claim 1 , wherein said composition further comprises a vehicle, optionally wherein said vehicle is selected from the group consisting of a liposome, nanoparticles, an immunostimulating complex (ISCOM), and slow-releasing particles.
5. The composition for use in a method of claim 4 , wherein said liposome comprises an emulsion, a foam, a micelle, an insoluble monolayer, a liquid crystal, a phospholipid dispersion, or a lamellar layer.
6. The composition for use in a method of claim 1 , wherein said tumor cells are part of a carcinoma.
7. The composition for use in a method of claim 6 , wherein said tumor cells are part of a ovarian carcinoma.
8. A composition for use in a method of:
(a) eliciting a CTL response against tumor cells presenting an epitopic peptide SEQ ID NO: 04 by administration of a sufficient amount of SEQ ID NO: 04 linked through a non-conventional peptide bond to an additional peptide sequence and a stabilizing agent wherein said peptides are in a stable form in the presence of the stabilizing agent in a subject; or
(b) stimulating an immune response in an immunologically competent animal, said composition consisting of SEQ ID NO: 04 and a stabilizing agent capable of facilitating an immune response thereof.