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A method for inhibiting xanthine oxidase and for reducing uric acid levels using a composition obtained by culturing Lactobacillus rhamnosus in a medium. Also disclosed is a composition including a metabolite of Lactobacillus rhamnosus for reducing uric acid levels in a subject and a method for producing the composition.
1. A method for reducing uric acid levels in a subject, the method comprising culturing Lactobacillus rhamnosus in a medium to form a composition and administering the composition to a subject in need thereof in an amount effective for reducing uric acid levels, wherein the Lactobacillus rhamnosus is removed from the composition prior to the administering step.
2. The method of claim 1 , wherein the Lactobacillus rhamnosus is Lactobacillus rhamnosus I21 deposited under Accession No. DSM 28876.
3. The method of claim 1 , wherein the subject suffers from gout or hyperuricemia.
4. The method of claim 1 , wherein the composition is administered orally, intramuscularly, intradermally, intravenously, subcutaneously, intraperitoneally, intranasally, or mucosally.
5. The method of claim 1 , wherein the medium is de Man-Rogosa-Sharpe (MRS) broth, milk, or a juice.
6. The method of claim 1 , further comprising lyophilizing the composition to form a powder.
7. A method for producing a composition for reducing uric acid levels in a subject, the method comprising inoculating a medium with Lactobacillus rhamnosus , culturing the Lactobacillus rhamnosus in the medium, removing the Lactobacillus rhamnosus from the medium to form a composition free of Lactobacillus rhamnosus , and lyophilizing the composition, wherein the Lactobacillus rhamnosus is Lactobacillus rhamnosus I21 deposited under Accession No. DSM 28876.
8. The method of claim 7 , wherein the culturing is carried out for 2 days at 37° C.
9. The method of claim 8 , wherein a culture density prior to the removing step is 1×10 9 cells/ml.
10. A method for producing a composition for reducing uric acid levels in a subject, the method comprising inoculating a medium with Lactobacillus rhamnosus , culturing the Lactobacillus rhamnosus in the medium, removing the Lactobacillus rhamnosus from the medium to form a composition free of Lactobacillus rhamnosus , and sterilizing the composition by pasteurization, irradiation, autoclave, or filtration, wherein the Lactobacillus rhamnosus is Lactobacillus rhamnosus I21 deposited under Accession No. DSM 28876.