Anti-HLA monoclonal chimeric immunoglobulin, process and kit employing such a monoclonal chimeric immunoglobulin
A monoclonal chimeric immunoglobulin wherein the heavy chains and the light chains are human by nature in their constant parts, in particular, the heavy chain constant parts are chosen from the group formed of the heavy chain constant parts of an IgA, of an IgG or of an IgM and the light chain constant parts are chosen from the group formed of the kappa chains and the lambda chains, and the light chain and the heavy chain variable parts are chosen from the group formed of monoclonal antibodies specific to monomorphic epitopes of HLA class I antigens and monoclonal antibodies specific to monomorphic epitopes of HLA class II antigens. A process for standardization of the screening and for quantification of anti-HLA antibodies in a liquid medium is also described.
1. A monoclonal chimeric comprising formed of:
(i) two polypeptide chains, called heavy chains (H), of molecular weight from 40 kDa to 60 kDa, and
(ii) two polypeptide chains, called light chains (L), of molecular weight from 20 kDa to 30 kDa,
wherein
each heavy chain (H) comprises:
a heavy chain variable region (VH) of a monoclonal antibody chosen from the group consisting of monoclonal antibodies specific to monomorphic epitopes of HLA class II antigens, and
a heavy chain constant region (CH) of a human immunoglobulin chosen from the group consisting of IgAs, IgGs and IgMs,
and wherein
at least one light chain (L) consists of the sequence SEQ ID NO: 5, and
at least one heavy chain (H) is selected from the group consisting of SEQ ID NO: 6, SEQ ID NO: 7 and SEQ ID NO: 8.
2. The monoclonal antibody according to claim 1 , wherein the at least one heavy chain (H) consists of the sequence of SEQ ID NO: 6.
3. The monoclonal antibody according to claim 1 , wherein the at least one heavy chain (H) consists of the sequence of SEQ ID NO: 7.
4. The monoclonal antibody according to claim 1 , wherein the at least one heavy chain (H) consists of the sequence of SEQ ID NO: 8.
5. A kit for the quantification of anti-HLA antibodies in a liquid medium, said kit comprising the monoclonal chimeric immunoglobulin of claim 1 .