Method for treating bleeding disorders
The invention concerns glycosylated proteins having human factor VIII activity. In a preferred embodiment, the protein is glycosylated with oligosaccharides that include an alpha-(2,6)-linked sialic acid and a bisecting GlcNAc linked to a core beta-mannose.
1. A method of treating hemophilia A comprising administering to a human in need thereof a therapeutically effective amount of a pharmaceutical composition comprising an isolated glycosylated protein having factor VIII procoagulant activity comprising an N-linked oligosaccharide having an alpha-(2,6)-linked sialic acid and a pharmaceutically acceptable adjuvant.
2. A method of treating hemophilia A comprising administering to a human in need thereof a therapeutically effective amount of a pharmaceutical composition comprising a glycosylated protein having factor VIII procoagulant activity comprising the amino acid sequence of SEQ ID NO: 1 and an N-linked oligosaccharide having an alpha-(2,6)-linked sialic acid and a pharmaceutically acceptable adjuvant.