Control of cellulose biosynthesis by overexpression of a transcription factor
The invention relates to the over-expression of a transcription factor selected from the group consisting of MYB46, HAM1, HAM2, MYB112, WRKY11, ERF6, and any combination thereof in a plant, which can modulate and thereby modulating the cellulose content of the plant.
1. A plant comprising an isolated nucleic acid comprising a poplar xylem-specific secondary cell wall specific cellulose synthase 8 promoter segment operably linked to a nucleic acid segment encoding a plant transcription factor selected from the group consisting of MYB46, HAM1, HAM2, MYB112, WRKY11, ERF6, or a combination thereof, wherein the plant is a softwood plant or hardwood plant that has at least about 2% increased cellulose content compared to a wild type plant of same species that does not have the isolated nucleic acid.
2. The plant of claim 1 , wherein the isolated nucleic acid expresses increased levels of the plant transcription factor in the plant compared to a corresponding transcription factor gene naturally present in a wild type plant of the same species.
3. The plant of claim 1 , wherein the plant has increased levels of secondary wall cellulose compared to a wild type plant of the same species without the isolated nucleic acid.
4. The plant of claim 1 , wherein the plant is a transgenic plan a genetically modified plant, or a plant selectively bred to comprise the isolated nucleic acid.
5. The plant of claim 1 , wherein the plant transcription factor is MYB46.
6. A seed comprising an isolated nucleic acid comprising a poplar xylem-specific secondary cell wall specific cellulose synthase 8 promoter segment operably linked to a nucleic acid segment encoding a plant transcription factor selected from the group consisting of MYB46, HAM1, HAM2, MYB112, WRKY11, ERF6, or a combination thereof wherein the seed is a softwood seed or hardwood seed.
7. A plant biomass comprising secondary wall cellulose isolated from a plant comprising an isolated nucleic acid comprising a poplar xylem-specific secondary cell wall specific cellulose synthase 8 promoter segment operably linked to a nucleic acid segment encoding a plant transcription factor selected from the group consisting of MYB46, HAM1, HAM2, MYB112, WRKY11, ERF6, or a combination thereof wherein the plant is a softwood plant or hardwood plant that has at least about 2% increased cellulose content compared to a wild type plant of the same species that does not have the isolated nucleic acid.
8. A method of increasing cellulose content in a plant comprising expressing MYB46, HAM1, HAM2, MYB112, WRKY11, ERF6, or any combination thereof in the plant from an isolated nucleic acid comprising a poplar xylem-specific secondary cell wall specific cellulose synthase 8 promoter segment operably linked to a nucleic acid segment encoding the transcription factor wherein the plant is a softwood plant or hardwood plant, and the plant has at least about 2% increased cellulose content compared to a wild type plant of the same species that does not have the isolated nucleic acid.
9. The plant of claim 1 , which is a poplar species, pine species, or eucalyptus species plant.
10. The seed of claim 6 , which is a poplar species, pine species, or eucalyptus species seed.