Yeast cell wall particles for receptor-targeted nanoparticle delivery
The present invention generally relates to yeast cell wall microparticles loaded with nanoparticles for receptor-targeted nanoparticle delivery. In particular, the present invention relates to trapping nanoparticles either on the surface or inside a yeast glucan particles, for example, yeast glucal particles. The present invention further relates to methods of making the yeast cell wall particles loaded with nanoparticles. The present invention also relates to methods of using the yeast cell wall particles loaded with nanoparticles for receptor-targeted delivery of the nanoparticles, e.g., drug containing nanoparticles.
1. A yeast cell wall particle-nanoparticle (YCWP-NP) delivery system, comprising yeast cell wall particles (YCWPs) comprising nanoparticles (NPs) having at least one dimension of about 10-1000 nm on the exterior surface of the YCWPs, wherein the YCWP delivery system is prepared by a process comprising the steps of:
(a) loading the NPs onto the YCWPs, wherein the loading comprises incubating a suspension comprising YCWPs and NPs for a time sufficient for the NPs to bind to the YCWPs, wherein the YCWPs have a trapping polymer covalently linked to the surface of the YCWPs, and wherein the YCWPs are surface-derivatized to have an electrostatic surface such that NP binding is facilitated,
(b) washing and/or dispersing the material resulting from (a) and
(c) optionally repeating steps (a)-(b) at least once,
such that the YCWP-NP delivery system is prepared.
2. The YCWP-NP delivery system of claim 1 , wherein the trapping polymer is selected from the group consisting of polyethyleneamine (PEI), polylysine (PLL), alginate, dextran sulfate and chitosan.
3. The YCWP-NP delivery system of claim 1 , wherein the NPs are selected from the group consisting of quantum dots, gold particles and magnetic particles.
4. The YCWP-NP delivery system of claim 1 , wherein the NPs are insoluble in water.
5. The YCWP-NP delivery system of claim 1 , wherein the NPs have at least one dimension of about 10-100 nm.
6. The YCWP-NP delivery system of claim 1 , wherein the NPs have at least one dimension of about 100-500 nm.
7. The YCWP-NP delivery system of claim 1 , wherein the NPs have at least one dimension of about 500-1000 nm.
8. The YCWP-NP delivery system of claim 1 , wherein the YCWPs are selected from the group consisting of YGPs, YGMPs, YCPs and YGCPs.
9. The YCWP-NP delivery system of claim 1 , wherein the YCWPs are YGPs.
10. The YCWP-NP delivery system of claim 1 , wherein the loading comprises incubating the suspension for about 12-24 hours.
11. A method of using the YCWP-NP delivery system of claim 1 in drug delivery.
12. A method of using the YCWP-NP delivery system of claim 1 in medical imaging.
13. A method for making a YCWP-NP delivery system comprising yeast cell wall particles (YCWPs) comprising nanoparticles (NPs) having at least one dimension of about 10-1000 nm on the exterior surface of the YCWPs, comprising the steps of:
(a) loading the NPs onto the YCWPs, wherein the loading comprises incubating a suspension comprising YCWPs and NPs for a time sufficient for the NPs to bind to the YCWPs, wherein the YCWPs have a trapping polymer covalently linked to the surface of the YCWPs, and wherein the YCWPs are surface-derivatized to have an electrostatic surface such that NP binding is facilitated,
(b) washing and/or dispersing the material resulting from (a) and
(c) optionally repeating steps (a)-(b) at least once,
such that the YCWP-NP delivery system is prepared.
14. The method of claim 13 , wherein the NPs are insoluble in water.
15. The method of claim 13 , wherein the NPs have at least one dimension of about 10-100 nm.
16. The method of claim 13 , wherein the NPs have at least one dimension of about 100-500 nm.
17. The method of claim 13 , wherein the NPs have at least one dimension of about 500-1000 nm.
18. The method of claim 13 , wherein the YCWPs are selected from the group consisting of YGPs, YGMPs, YCPs and YGCPs.
19. The method of claim 13 , wherein the YCWPs are YGPs.
20. The method of claim 13 , wherein the loading comprises incubating the suspension for about 12-24 hours.
21. The method of claim 13 , wherein the YCWP-NP delivery system is used in drug delivery.
22. The method of claim 13 , wherein the YCWP-NP delivery system is used in medical imaging.