Laccases for bio-bleaching
Provided herein are isolated laccase enzymes and nucleic acids encoding them. Also provided are mediators for laccase reactions. Also provided herein are methods for using laccases to oxidize lignins, other phenolic, and aromatic compounds, such as for bio-bleaching and decolorization of wood pulp under high temperature and pH conditions to facilitate a substantial reduction in use of bleaching chemicals, as well as for treatment of fibers.
1. An isolated polynucleotide, comprising: a nucleic acid sequence comprising 80%-99% sequence identity with the nucleic acid sequence of SEQ ID NO: 1, wherein said polynucleotide encodes a polypeptide comprising laccase activity, oxidizes lignin under conditions of pH greater than or equal to pH 8.0, and retains laccase activity for at least about 5 minutes at greater than or equal to 60° C.
2. The isolated polynucleotide of claim 1 , wherein said polynucleotide comprises a sequence that shares at least 90% sequence identity with SEQ ID NO: 1.
3. The isolated polynucleotide of claim 1 , wherein said polynucleotide comprises a sequence that shares at least 95% sequence identity with SEQ ID NO: 1.
4. The isolated polynucleotide of claim 1 , wherein said polynucleotide comprises a sequence that shares at least 97% sequence identity with SEQ ID NO: 1.
5. The isolated polynucleotide of claim 1 , wherein said polynucleotide comprises a sequence that shares at least 99% sequence identity with SEQ ID NO: 1.