IP Library › Granted Patent US 9,678,062
Granted Patent B2
US 9,678,062 · App. 14/346,505 · Granted Jun 13, 2017

Methods and compositions for expanding cells and improving engraftment

Inventors: Suzanne T. Ildstad (Prospect, KY); Mary Jane Elliott (Brandenburg, KY)
Assignee: University of Louisville Research Foundation, Inc.
G01N33/5073C12N5/0639G01N33/5029C12N2501/998C12N2502/1171
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Quick Facts
Patent No.
US 9,678,062
App. No.
14/346,505
Granted
Jun 13, 2017
Kind
B2
Abstract

Methods of expanding FCs or stem cells are provided herein, as are methods of screening for compounds that increase expression of DOCK2 or decrease expression of Arhgap18, either of which improves engraftment.

Claims (7)

1. A method of enhancing the function of CD8+/TCR− facilitating cells, comprising the step of:

contacting said facilitating cells (FCs) ex vivo with exogenous DOCK2 polypeptides.

2. The method of claim 1 , wherein the DOCK2 polypeptides are human DOCK2 polypeptides.

3. The method of claim 1 , wherein the DOCK2 polypeptides are recombinant DOCK2 fusion polypeptides.

4. The method of claim 1 , wherein the FCs are purified.

5. The method of claim 1 , wherein the function of the FCs comprises in vitro migration to SDF-1, in vitro induction of T regulatory cells (Treg), in vitro induction of Tr1 cells, enhancing hematopoietic stem cell (HSC) homing, enhancing HSC lodgment, or preventing HSC apoptosis.

6. The method of claim 5 , wherein the ability of the FCs to enhance HSC homing and/or enhance HSC lodgment is determined using an in vivo syngeneic homing model followed by a CFC assay.

Assignments (2)
ASSIGNMENT OF ASSIGNOR'S INTEREST Recorded Jan 8, 2015
From: ILDSTAD, SUZANNE T.; ELLIOTT, MARY JANE
To: UNIVERSITY OF LOUISVILLE RESEARCH FOUNDATION, INC.
Reel/Frame 034661/0815 →
CONFIRMATORY LICENSE Recorded Jul 1, 2014
From: UNIVERSITY OF LOUISVILLE RESEARCH FOUNDATION
To: US ARMY, SECRETARY OF THE ARMY
Reel/Frame 033259/0157 →
Continuity (2)
Provisional Application 61538633 · Sep 23, 2011
Related Publication 20140234843A1 · Aug 21, 2014