Materials for magnetizing cells and magnetic manipulation
A material comprising positively and negatively charged nanoparticles, wherein one of said nanoparticles contained a magnetically responsive element, are combined with a support molecule, which is a long natural or synthetic molecule or polymer to make a magnetic nanoparticle assembly. When the magnetic nanoparticle assembly is combined with cells, it will magnetize those cells. The magnetized cells can then be washed to remove the magnetic nanoparticle assembly and the magnetized cells manipulated in a magnetic field.
1. A method of growing a 3D cell culture, said method comprising:
a) mixing cells with a composition comprising:
i) a negatively charged nanoparticle;
ii) a positively charged nanoparticle; and
iii) a support molecule;
iv) wherein one of said negatively charged nanoparticle or positively charged nanoparticle contains a magnetically responsive element, and wherein said support molecule holds said negatively charged nanoparticle and said positively charged nanoparticle in an intimate admixture forming a fibrous mat-like structure;
b) incubating said mixture for 1 to 12 hours until the cells become magnetized;
c) subjecting said magnetized cells to a magnetic field sufficient to levitate said magnetized cells; and
d) growing said levitated cells to make a levitated 3D culture.
2. The method of claim 1 , wherein said negatively charged nanoparticle is a gold nanoparticle.
3. The method of claim 1 , wherein said positively charged nanoparticle is an iron oxide nanoparticle.
4. The method of claim 1 , wherein the support molecule comprises peptides, polysaccharides, nucleic acids, polymers or combinations thereof.
5. The method of claim 1 , wherein the support molecule comprises poly-lysine, fibronectin, collagen, laminin, BSA, hyaluronan, glycosaminoglycan, anionic, non-sulfated glycosaminoglycan, gelatin, nucleic acid, extracellular matrix proteins, cell extract, antibody or mixtures or derivatives thereof.
6. The method of claim 1 , wherein the support molecule comprises poly-lysine.
7. The method of claim 1 , wherein said composition comprises FeO 2 —Au-poly-lysine.
8. The method of claim 1 , wherein said magnetized cells are washed before being levitated.
9. The method of claim 1 , wherein said cells are a mixture of more than one cell type.
10. The method of claim 1 , wherein in step c) said magnetized cells are subject to a magnetic field which provides a specific shape sufficient to levitate and shape said magnetized cells; and in step d) the levitated cells are shaped by growing said levitated and shaped cells to make a levitated 3D culture having said shape.
11. The method of claim 10 , wherein said magnetic field provides shapes which are: toroidal, spherical, circular, polygonal or line shaped and wherein said levitated 3D culture is toroidal, spherical, circular, polygonal or line shaped.
12. The method of claim 11 , wherein a plurality of toroidal 3D cultures are stacked one on top of another to make a tubular 3D culture.
13. The method of claim 10 , wherein said magnetic field provides shapes which are: toroidal, spherical, circular rectangular, or line shaped and wherein said levitated 3D culture is toroidal.