Salivary biomarkers for lung cancer detection
Presented herein are biomarkers related to lung cancer. The presently identified salivary biomarkers create the basis for a lung cancer detection bioassay with sensitivity and specificity. Means and methods for evaluating the data generated using multiple biomarkers in order to validate findings and further use of the multiplexed lung cancer assay in clinical, diagnostic and therapeutic uses is also included.
1. A method for diagnosing the presence of lung cancer in a subject at risk of having lung cancer, the method comprising: a) contacting a saliva sample from the subject with a set of at least three reagents, wherein the set of at least three reagents specifically binds to at least three biomarkers in the saliva sample, wherein the three biomarkers are selected from the group consisting of BRAF, EGFR, FGF19, LZTS1, CCNI, GREB1, and FRS2; b) measuring the level of the at least three biomarkers in the saliva sample; c) detecting that the level of the at least three biomarkers is increased in the saliva sample relative to a control sample from a subject without lung cancer, thereby detecting the presence of lung cancer in the subject; and d) administering a lung cancer therapy to the subject, wherein the lung cancer therapy is selected from the group consisting of chemotherapy, hormonal therapy, radiotherapy, immunotherapy, and surgical removal of lung tissue.
2. The method of claim 1 , wherein the assay detects at least three nucleic acids encoding at least three biomarkers, and wherein the at least three nucleic acids are detected by mass spectroscopy, PCR, microarray hybridization, thermal sequencing, capillary array sequencing, or solid phase sequencing.
3. The method of claim 1 , wherein the assay that specifically detects at least three biomarkers in the saliva sample, detects at least four biomarkers selected from the group consisting of BRAF, EGFR, FGF19, LZTS1, CCNI, GREB1, and FRS2.
4. The method of claim 1 , wherein the assay that specifically detects at least three biomarkers in the saliva sample, detects at least five biomarkers selected from the group consisting of BRAF, EGFR, FGF19, LZTS1, CCNI, GREB1, and FRS2.