IP Library › Granted Patent US 9,696,309
Granted Patent B2
US 9,696,309 · App. 14/408,393 · Granted Jul 4, 2017

Methods of measuring levels of phosphorylated neuronal nitric oxide synthase

Inventors: Irina Burd (Silver Spring, MD); Talaibek Borbiev (Rockville, MD); Elisabeth Nigrini (Baltimore, MD); Michael Johnston (Baltimore, MD)
Assignees: The Johns Hopkins University; Kennedy Krieger Institute, Inc.
G01N33/573G01N33/535G01N33/6896G01N2333/90254G01N2440/14G01N2800/28
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Quick Facts
Patent No.
US 9,696,309
App. No.
14/408,393
Granted
Jul 4, 2017
Kind
B2
Abstract

The present invention relates to the field of biomarkers. More specifically, the present invention relates to biomarkers useful in diagnosing brain injuries. In a specific embodiment, a method for diagnosing perinatal brain injury in a patient comprises the steps of (a) determining the ratio of phosphorylated nNOS to unphosphorylated nNOS in a sample collected from the patient using an ELISA; and (b) comparing the ratio with predefined ratios of the same proteins that correlate to a patient having perinatal brain injury and predefined ratios of the same proteins that correlate to a patient not having perinatal brain injury, wherein a correlation to one of the predefined ratios provides the diagnosis.

Claims (2)

1. A method comprising the step of measuring the levels of phosphorylated neuronal nitric oxide synthase (nNOS) (p-NOS) and total nNOS (pan-NOS) by performing an immunoassay with either (a) an amniotic fluid sample obtained from a pregnant female or (b) a cord blood sample obtained from a neonate, wherein the p-NOS is phosphorylated at Ser852 of SEQ ID NO:1.

2. The method of claim 1 , wherein the immunoassay is an enzyme-linked immunosorbent assay (ELISA).

Assignments (2)
ASSIGNMENT OF ASSIGNOR'S INTEREST Recorded May 30, 2017
From: BURD, IRINA; BORBIEV, TALAIBEK; NIGRINI, ELISABETH
To: THE JOHNS HOPKINS UNIVERSITY
Reel/Frame 042524/0245 →
ASSIGNMENT OF ASSIGNOR'S INTEREST Recorded May 30, 2017
From: JOHNSTON, MICHAEL
To: THE JOHNS HOPKINS UNIVERSITY; KENNEDY KRIEGER INSTITUTE, INC.
Reel/Frame 042524/0333 →
Continuity (3)
Provisional Application 61567882 · Dec 7, 2011
Provisional Application 61668654 · Jul 6, 2012
Related Publication 20150247854A1 · Sep 3, 2015