Stem cell separating material and method of separation
The present invention has its object to provide a material for separating stem cell and a filter for separating stem cell, each is capable of selectively separating and recovering, in a simple and easy manner, stem cells from body fluids or biological tissue-derived treated fluids, a method for separating and recovering stem cells, and stem cells obtained by such method. The present invention is a material for separating stem cell which has a density K of 1.0×10 4 ≦K≦1.0×10 6 and a fiber diameter of 3 to 40 μm; a filter for separating stem cell which comprises the material for separating stem cell as packed in a container having a fluid inlet port and a fluid outlet port; a method of separating and recovering stem cells which comprises using the material for separating stem cell or the filter for separating stem cell; and a method of producing a multipotent cell fraction.
1. A filter for separating stem cells which comprises a material for separating stem cells which has a density K of 1.0×10 4 g/m 3 <K<1.0×10 6 g/m 3 and a fiber diameter of 5 um to 30 um,
which is made of combination of fibers of two or more synthetic polymer species in combination, and having mesh openings which are substantial pores formed by crossing two or more different fibers,
wherein a minor axis of each mesh opening is not shorter than 5 μm and a major axis thereof is not longer than 80 μm, and
which has the form of a nonwoven fabric,
wherein the combination of fibers of two or more synthetic polymer species is a combination of rayon and a polyolefin, and wherein the material for separating stem cells is packed in a container having a fluid inlet port and a fluid outlet port.
2. The filter for separating stem cells according to claim 1 , wherein the fibers of synthetic polymer species is split fiber or conjugated fiber.
3. The filter for separating stem cells according to claim 1 , which has a density K of 1.0×10 4 <K<2.0×10 5 g/m 3 .
4. The filter for separating stem cells according to claim 1 , wherein a washing fluid inlet is provided at the fluid inlet port or on the fluid inlet side other than the fluid inlet port for washing away unnecessary cells and other unnecessary substances remaining in the material for separating stem cell and a cell recovering fluid inlet is provided at the fluid outlet port or at a site on the fluid outlet side other than the fluid outlet port for recovering cells captured by the material for separating stem cells.
5. The filter for separating stem cells according to claim 4 wherein a bag for receiving and storing a cell recovering fluid containing cells captured by the material for separating stem cells is provided at the fluid inlet port or washing fluid inlet or at a site on the fluid inlet side other than the fluid inlet port or washing fluid inlet.
6. The filter for separating stem cells according to claim 5 , wherein the bag for receiving and storing a cell recovering fluid containing cells captured by the material for separating stem cells is a bag for cell culture.