Inorganic-binding peptides and quality control methods using them
The present invention relates to quality control methods using inorganic binding peptides, wherein inorganic entities are identified using inorganic-binding peptides specifically binding to the inorganic entity of interest. In particular, the invention includes a method for the identification of defects or inhomogeneities on a surface by detecting an inorganic entity of interest. It further includes a method for the isolation of powder particles comprising an inorganic entity of interest from a mixture of powder particles. In addition, the present invention relates to inorganic-binding peptides comprising the amino acid sequence MTWDPSLASPRS (SEQ ID NO: 31) and the amino acid sequence LNAAVPFTMAGS (SEQ ID NO: 32), respectively.
1. A method of separating inorganic powder particles from a mixture of powder particles, comprising:
(a) contacting the mixture of powder particles, wherein the mixture of powder particles comprises stainless steel powder particles and at least one different inorganic powder particle, with an inorganic-binding peptide having SEQ ID NO: 31 attached to a solid support, wherein the stainless steel powder particles bind to the inorganic-binding peptide having SEQ ID NO: 31; or
contacting the mixture of powder particles, wherein the mixture of powder particles comprises titanium dioxide (TiO 2 ) powder particles and at least one different inorganic powder particle, with an inorganic-binding peptide having SEQ ID NO: 32 attached to a solid support, wherein the TiO 2 powder particles bind to the inorganic-binding peptide having SEQ ID NO: 32; and
(c) separating the stainless steel powder particles bound to the inorganic-binding peptide having SEQ ID NO: 31 attached to the solid support from the at least one different inorganic powder particle; or
separating the TiO 2 powder particles bound to the inorganic-binding peptide having SEQ ID NO: 32 attached to the solid support, wherein the at least one different inorganic powder particle comprises at least one material selected from the group consisting of aluminum, antimony, beryllium, cadmium, copper, chrome, gold, iron, lead, selenium, palladium, platinum, stainless steel, titanium, zinc, silicon, germanium, carbon, and oxides thereof.
2. The method of claim 1 , wherein the solid support is a magnetic bead.
3. The method of claim 1 , further comprising c) removing the inorganic binding peptide from the stainless steel powder particles, or removing the inorganic binding peptide from the TiO 2 powder particles.
4. The method of claim 1 , wherein the mixture of powder particles comprise a nanopowder.