Regulatory sequence of cupin family gene
This invention is in the field of plant biology and agriculture and relates to novel seed specific promoter regions. The present invention further provide methods of producing proteins and other products of interest and methods of controlling expression of nucleic acid sequences of interest using the seed specific promoter regions.
1. An isolated DNA molecule comprising a plant regulatory element, wherein the regulatory element comprises SEQ ID NO: 3, and a heterologous gene operably linked to said regulatory element.
2. An expression vector, comprising the DNA molecule of claim 1 .
3. A transgenic plant cell, comprising the DNA molecule of claim 1 .
4. A transgenic plant, comprising the DNA molecule of claim 1 .
5. A transgenic plant embryo, comprising the DNA molecule of claim 1 .
6. A plant gene expression cassette comprising, in sequence, a regulatory element comprising SEQ ID NO: 3 operably linked to the coding region of a heterologous gene and a 3′ polyadenylation signal.
7. A method for selectively expressing a nucleotide sequence in a plant embryo, the method comprising transforming a plant embryo with a transformation vector comprising an expression cassette, the expression cassette comprising a regulatory element and a heterologous nucleotide sequence operably linked to the regulatory element, said regulatory element comprising the sequence of SEQ ID NO: 3.
8. A method of producing a protein of interest in a plant embryo, comprising: a) providing a transgenic embryo comprising a heterologous nucleic acid sequence encoding the protein of interest operably linked to a regulatory element, wherein the regulatory element comprises SEQ ID NO:3; and b) growing the plant under conditions such that the protein is produced in said embryo.
9. The method of claim 8 , wherein said embryo is a monocot embryo.
10. The method of claim 8 , wherein said producing a protein corresponds with the embryonic stage of plant development.