Method for protecting kidney function
The present invention relates to a method for protecting a kidney, which comprises administering an extracellular polysaccharide produced by Ceriporia lacerata ; a mycelial culture medium of Ceriporia lacerata containing the extracellular polysaccharide; dried powders of the mycelial culture medium; or an extract of the mycelial culture medium, in an effective amount, to a subject in need of protecting a kidney. The method is effective in protecting a kidney from renal toxicity caused by an anti-cancer agent.
1. A method for protecting a kidney, which comprises administering an extracellular polysaccharide produced by Ceriporia lacerata ; a mycelial culture medium of Ceriporia lacerata containing the extracellular polysaccharide; dried powders of the mycelial culture medium; or an extract of the mycelial culture medium, in an effective amount, to a subject in need of protecting a kidney.
2. The method of claim 1 , wherein the extracellular polysaccharide comprises 40 to 60 wt % sugar and 30 to 40 wt % protein, and has a molecular weight of 100 to 150 kDa.
3. The method of claim 2 , wherein the extracellular polysaccharide comprises 43 to 47 wt % sugar and 33 to 36 wt % protein, and has a molecular weight of 115 to 125 kDa.
4. The method of claim 2 , wherein the sugar contains mannose, galactose and glucose.
5. The method of claim 1 , wherein the extracellular polysaccharide is prepared by the preparation method comprising the steps of:
(a) culturing the mycelia of Ceriporia lacerata in a liquid to prepare a mycelial culture medium of Ceriporia lacerata;
(b) drying the mycelial culture medium of Ceriporia lacerata to form powders; and
(c) extracting the powders of the mycelial culture medium of Ceriporia lacerata with a solvent, and filtering and concentrating the resultant extract under reduced pressure.
6. The method of claim 5 , wherein the medium for culturing in a liquid comprises sugar, glucose, starch, sorghum flour, barley powder, soybean flour, magnesium sulfate (MgSO 4 ), monopotassium phosphate (KH 2 PO 4 ), dipotassium phosphate (K 2 HPO 4 ) and water, and the hydrogen ion concentration (pH) of the medium is 4.5 to 6.0.
7. The method of claim 5 , wherein the culturing in a liquid is conducted under a blue LED light source.
8. The method of claim 5 , wherein the culturing in a liquid is conducted with a carbon dioxide concentration at 1,000 to 2,000 ppm.
9. The method of claim 1 , wherein the subject in need of protecting a kidney is a patient whose kidney is damaged or shows impaired function due to anti-cancer therapy.
10. The method of claim 9 , wherein the anti-cancer therapy is anti-cancer agent therapy.
11. The method of claim 10 , wherein the anti-cancer agent is a platinum-based anti-cancer agent.
12. The method of claim 11 , wherein the platinum-based anti-cancer agent is selected from the group consisting of cisplatin, carboplatin, oxaliplatin, nedaplatin and a mixture thereof.
13. The method of claim 1 , wherein the extracellular polysaccharide produced by Ceriporia lacerata ; the mycelial culture medium of Ceriporia lacerata containing the extracellular polysaccharide; the dried powders of the mycelial culture medium; or the extract of the mycelial culture medium is administered in the form of a pharmaceutical composition which further contains a pharmaceutically acceptable excipient.
14. The method of claim 1 , wherein the extracellular polysaccharide produced by Ceriporia lacerata ; the mycelial culture medium of Ceriporia lacerata containing the extracellular polysaccharide; the dried powders of the mycelial culture medium; or the extract of the mycelial culture medium is administered in the form of a health functional food which further contains a sitologically acceptable excipient.
15. The method of claim 9 , wherein the extracellular polysaccharide produced by Ceriporia lacerata ; the mycelial culture medium of Ceriporia lacerata containing the extracellular polysaccharide; the dried powders of the mycelial culture medium; or the extract of the mycelial culture medium is administered in the form of a pharmaceutical composition which further contains a pharmaceutically acceptable excipient.
16. The method of claim 1 , wherein the subject in need of protecting a kidney is a patient receiving anti-cancer therapy, and the extracellular polysaccharide produced by Ceriporia lacerata ; the mycelial culture medium of Ceriporia lacerata containing the extracellular polysaccharide; the dried powders of the mycelial culture medium; or the extract of the mycelial culture medium is administered to the patient in combination with the anti-cancer therapy.