Application and uses of PRG4 and therapeutic modulation thereof
The present invention relates to the uses of the protein PRG4 and therapeutic modulation thereof. In particular, the present invention relates compositions and methods utilizing PRG4 and therapeutic modulation thereof, including, use as a surgical lubricant, use in a treatment for prevention or reduction of post-surgical adhesions, use in a treatment for oral ulcerations, use as an athletic lubricating patch, use as a dermal filler, use in a treatment for dry mouth, use in a drug delivery method or composition, and use in nursing lubrication.
1. A method for treating decreased oral cavity boundary lubrication or disorder or symptoms associated therewith, or a improving oral cavity boundary lubrication in a patient in need thereof, comprising administering to the patient a composition comprising an effective amount or concentration of proteoglycan 4 (PRG4) having at least 95% primary amino acid sequence homology with the amino acid sequence of SEQ ID NO:1.
2. The method of claim 1 , wherein the PRG4 is formulated in an aqueous, osmotically balanced salt solution; multiphasic emuslion; gel; liquid; cream; ointment; spray; paste; viscous solution; slow-release device; lozenge; or gum.
3. The method of claim 1 , wherein the disorder or symptoms associated with decreased oral cavity boundary lubrication are selected from the group consisting of oral ulceration, xerostomia, Sjogren's syndrome; allergy; oral surface disorders; chronic inflammation; hyperosmolarity; aging; or nerve damage; side effects associated with prescription or over-the-counter drugs, radiation therapy, or chemotherapy; and any combinations thereof.
4. The method of claim 1 , wherein the composition further comprises one or more of:
(a) sodium hyaluronate or hyaluronic acid;
(b) about 0.25 wt. % to 10 wt. % eriodictyon fluid extract;
(c) about 1.0 wt. % to 30 wt. % sweetener or flavoring agent;
(d) a stimulator compound selected from the group consisting of citric acid, ascorbic acid, and mixtures thereof, in an amount effective to stimulate salivary gland secretion;
(e) one or more local anaesthetics selected from the group consisting of lidocaine, lignocaine, and prilocalne; and/or
(f) one or more enzymes found in saliva selected from the group consisting of lactoperoxidase, thiocyanate, or glucoseoxidase.
5. The method of claim 1 , wherein the composition futher comprising a gum base and one of more of:
(a) about 0.25 wt. % to 10 wt. % eriodictyon fluid extract, citrus oil, lemon oil, lime oil, neroli oil, orange oil, a mint oil, peppermint oil, spearmint oil, anise oil, cardamom oil, cinnamon oil, clove oil, coriander oil, eucalyptus oil, fennel oil, lemongrass oil, nutmeg oil, eriodictyon fluid extract, or glycyrrhiza extract;
(b) about 1.0 wt. % to 30 wt. % sweetener or flavoring agent;
(c) a stimulator compound selected from the group consisting of citric acid, ascorbic acid, and mixtures thereof, in an amount effective to stimulate salivary gland secretion; and
(d) a therapeutically effective amount of sodium hyaluronate.
6. The method of claim 1 , wherein the composition is a lozenge composition comprising a shaped solid, the comprising further comprising one or more of the following:
(a) about 0.25 wt. % to 10 wt. % eriodictyon fluid extract;
(b) about 1.0 wt. % to 30 wt. % sweetener;
(c) a stimulator compound selected from the group consisting of citric acid, ascorbic acid, and mixtures thereof, in an amount effective to stimulate salivary gland secretion; and
(d) a gelatin base; and
(e) a therapeutically effective amount of sodium hyaluronate.
7. The method of claim 1 , wherein the PRG4 is at a concentration of 10-100,000 μg/mL.
8. The method of claim 1 , wherein the PRG4 is an isolated or purified native PRG4.
9. The method of claim 1 , wherein the PRG4 is a recombinant PRG4.
10. The method of claim 1 , wherein the PRG4 is human PRG4.
11. The method of claim 1 , wherein the PRG4 has the amino acid sequence of SEQ ID NO:1.
12. The method of claim 4 , wherein the sodium hyaluronate or hyaluronic acid is at a concentration of 10-100,000 μg/mL.