Method for embedding a biological sample in a transparent matrix for analysis using single plane illumination microscopy
The invention is directed to method for positioning and aligning a preferably biological sample in the detection area of the objective of a microscope arrangement. According to the invention, the method mentioned above has the following method steps: a sample is introduced into a transparent medium, preferably agarose gel, which is initially liquid; the medium is changed from the liquid state to the solid state, wherein the sample is fixated within the medium, but the transparency of the medium is retained; the solidified medium is positioned in the microscope arrangement in such a way that the sample enclosed therein is situated in the detection area of the objective. Further, a device is proposed for positioning and aligning a preferably biological sample in the detection area of the objective of a microscope arrangement.
1. A device for introducing a sample into a detection area of an objective of a microscope arrangement, comprising:
a reservoir for a transparent medium which is initially still liquid;
a needle-piston unit comprising:
a hollow needle; and
a suction and delivery piston which is movably guided in the needle for sucking a partial amount of the medium into the hollow needle or ejecting it from the hollow needle; and
a manipulating unit comprising:
a receptacle configured to receive the needle-piston unit; and
a connection portion configured to connect to a holder of the microscope arrangement;
wherein the manipulating unit is configured to manipulate the needle-piston unit so as to:
position and align the partial amount of medium, after the partial amount of medium is solidified within the needle, in the microscope arrangement in such a way that a sample contained in the solidified partial amount of medium is situated in the detection area of the objective; and
change a position of the sample along a coordinate X, a coordinate Y, and a coordinate Z; and
rotate the sample around a longitudinal direction of the hollow needle by an angle φ;
wherein the needle-piston unit is fastened to the manipulating unit by means of connection elements configured to be manually disconnected from the hollow needle;
wherein the hollow needle of the needle-piston unit is in the form of a capillary or a cannula.
2. The device according to claim 1 , further comprising:
a means for introducing the sample into the partial amount medium after the partial amount of medium is arranged in the needle.