IP Library › Granted Patent US 9,738,893
Granted Patent B2
US 9,738,893 · App. 15/004,104 · Granted Aug 22, 2017

Method of enhancing miR-185 expression to reduce low density lipoprotein/cholesterol accumulation in a cell

Inventors: Muhua Yang (Hamilton, NJ); Joseph T. Nickels (Robbinsville, NJ); Weidong Liu (Chesterbrook, PA); Christina Ann Pellicane (Edison, NJ)
Assignee: Medical Diagnostic Laboratories, LLC
C12N15/113C12N15/1137C12N15/1138C12Q1/6883C12N2310/141C12Q2600/158C12Q2600/178C12Y101/01034C12Y205/01021
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Quick Facts
Patent No.
US 9,738,893
App. No.
15/004,104
Granted
Aug 22, 2017
Kind
B2
Abstract

The present invention provides a method of quantifying miR-185 as a potential biomarker in lipid disorder or cardiovascular diseases in human. The present invention also provides a method of modulating miR-185 in regulating LDL and cholesterol metabolism in cells. The present invention has therapeutic potential in the treatment of cholesterol/LDL related cardiovascular diseases in humans.

Claims (5)

1. A method for reducing the expression level of SREBP-2 in a cell comprising the steps of:

a) providing a cell in need of reducing the expression level of SREBP-2; and

b) transfecting in said cell with a composition comprising a miR-185 precursor, said miR-185 precursor consisting of a nucleotide sequence set forth in SEQ ID NO: 1, wherein said transfecting step of said miR-185 precursor causes the inhibition of SREBP-2 in said cell.

2. The method of claim 1 , wherein said SREBP-2 is a mRNA or a protein.

3. The method of claim 1 , wherein said SREBP-2 is a mRNA.

Assignments (1)
SECURITY INTEREST Recorded Apr 27, 2018
From: MEDICAL DIAGNOSTIC LABORATORIES, L.L.C.
To: TD BANK, N.A.
Reel/Frame 046031/0381 →
Continuity (3)
Continuation 13919141 · Jun 17, 2013
Provisional Application 61660057 · Jun 15, 2012
Related Publication 20160194635A1 · Jul 7, 2016